Product Description: 3-oxoacyl-[acyl-carrier-protein] reductase
SignalP Peptide: MSVLHRFYLFFLFTKFFHC
# Transmembrane Domains: 0
EC Numbers: 1.1.1.100 (3-oxoacyl-[acyl-carrier-protein] reductase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0070402 | NADPH binding |
| Function | GO:0003723 | RNA binding |
| Function | GO:0018454 | acetoacetyl-CoA reductase activity |
| Function | GO:0016616 | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| Process | GO:0055114 | oxidation-reduction process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | possibly expressed | 0.02 | 0.99 |
| Schizont | possibly expressed | 0.00 | 1.00 |
| Gametocyte | expressed | 0.31 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0922900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.919 | MFS: -2.588 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0823800)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0823800)
Modification: Disrupted | RMgm-4608
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2S7
"Best" AlphaFill ligand hit: NAP (nadp nicotinamide-adenine-dinucleotide phosphate, Local RMSD=0.06) with 4CQM (Global RMSD=1.43)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.1.1.100 | 3-oxoacyl-[acyl-carrier-protein] reductase | RNAiNADP+quercetinbithionolkaempferolresveratrolTannic acidtachyplesinPhenylglyoxalanthecotulideHexachlorophenebromochlorophen... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8I2S7 | 3-oxoacyl-[acyl-carrier-protein] reductase | Plasmodium falciparum (isolate 3D7) | HOGENOM, OMA, OrthoDB, OrthoMCL, OrthoMCL BLAST | Epigallocatechin 3-gallate |
| Q581W1 | Pteridine reductase, putative | OrthoDB | MethotrexateCHEMBL1232702CHEMBL1232399CHEMBL2178602... | |
| Q965D6 | 3-oxoacyl-acyl-carrier protein reductase | Plasmodium falciparum | OrthoMCL BLAST | CHEMBL1269411Epicatechin-3-O-gallateCHEMBL129451acs.jmedchem.1c00409_ST.600... |
Ortholog Group (OrthoMCL): OG6_100272
Most Similar Human Ortholog: Q8N4T8
TM-align score: 0.95 | RMSD: 1.27
Seq Identity: 0.38 | Length: 235 / 301
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000112474 | hydroxysteroid 17-beta dehydrogenase 8 |
| ENSG00000145439 | carbonyl reductase 4 |
| ENSG00000204228 | hydroxysteroid 17-beta dehydrogenase 8 |
| ENSG00000225312 | hydroxysteroid 17-beta dehydrogenase 8 |
| ENSG00000228357 | hydroxysteroid 17-beta dehydrogenase 8 |
| ENSG00000228712 | hydroxysteroid 17-beta dehydrogenase 8 |
| ENSG00000232357 | hydroxysteroid 17-beta dehydrogenase 8 |
Protein Length: 301 | Molecular Weight (kDa): 33.347
PDB ID(s): 2C07
Isoelectric Point: 9.7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002347 | Short-chain dehydrogenase/reductase SDR |
| InterPro | IPR011284 | 3-oxoacyl-(acyl-carrier-protein) reductase |
| InterPro | IPR020904 | Short-chain dehydrogenase/reductase, conserved site |
| InterPro | IPR036291 | NAD(P)-binding domain superfamily |
| PFam | PF13561 | N/A |
| Superfamily | SSF51735 | NAD(P)-binding domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 16 | 5 | 26 |
| disruptive | 1 | 24 | 13 | 40 |
| missense | 1 | 24 | 13 | 40 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 21 | 12 | 26 |
| disruptive | 5 | 55 | 35 | 56 |
| missense | 5 | 49 | 35 | 48 |
PlasmoDB Total SNPs: 69
Non-coding: 56 | Synonymous: 7 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 12646215 | Functional characterization of beta-ketoacyl-ACP reductase (FabG) from Plasmodiumfalciparum. | Pillai S, Rajagopal C, ..., Surolia N | 10.1016/s0006-291x(03)00321-8 |
| 15939298 | Production and purification of refolded recombinant Plasmodium falciparumbeta-ketoacyl-ACP reductase from inclusion bodies. | Karmodiya K, Srivastav RK, Surolia N | 10.1016/j.pep.2005.02.008 |
| 16225460 | Kinetic, inhibition and structural studies on 3-oxoacyl-ACP reductase fromPlasmodium falciparum, a key enzyme in fatty acid biosynthesis. | Wickramasinghe SR, Inglis KA, ..., Fairlamb AH | 10.1042/BJ20050832 |
| 18721141 | Deciphering the key residues in Plasmodium falciparum beta-ketoacyl acyl carrierprotein reductase responsible for interactions with Plasmodium falciparum acylcarrier protein. | Karmodiya K, Modak R, ..., Surolia N | 10.1111/j.1742-4658.2008.06608.x |
| 24297444 | Type II fatty acid biosynthesis is essential for Plasmodium falciparum sporozoitedevelopment in the midgut of Anopheles mosquitoes. | van Schaijk BC, Kumar TR, ..., Vaughan AM | 10.1128/EC.00264-13 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 36216219 | Heterologous expression, purification, and partial characterisation of theapicoplast protein 3-oxoacyl-[acyl-carrier-protein] reductase from Toxoplasmagondii | Aygun C, Kocer S, ..., Mutlu O | 10.1016/j.pep.2022.106187 |