About Gene List

PF3D7_0923000 (RPB3)

Genome location: Pf3D7_09_v3:938,929..940,716(-)

Genome classification: Core

Function and Localization

Product Description: DNA-directed RNA polymerase II subunit RPB3, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005665 RNA polymerase II, core complex
Component GO:0005634 nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring possibly expressed 0.17 0.91
Trophozoite expressed 0.16 0.90
Schizont possibly expressed 0.02 0.99
Gametocyte possibly expressed 0.17 0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0923000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.258 | MFS: -2.555 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2S6

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.7.7.6 DNA-directed RNA polymerase BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101363

Most Similar Human Ortholog: H3BRR2

TM-align score: 0.86 | RMSD: 1.17

Seq Identity: 0.30 | Length: 43 / 335

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000102978 RNA polymerase II subunit C

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 0 12 8 32
disruptive 5 16 10 34
missense 4 10 10 31

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 2 33 15 22
disruptive 11 36 34 65
missense 6 33 27 48

PlasmoDB Total SNPs: 67

Non-coding: 54 | Synonymous: 9 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 335 | Molecular Weight (kDa): 38.346

UniProt ID(s): Q8I2S6

PDB ID(s): None

Isoelectric Point: 6.45

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001514 DNA-directed RNA polymerase, 30-40kDa subunit, conserved site
InterPro IPR011262 DNA-directed RNA polymerase, insert domain
InterPro IPR011263 DNA-directed RNA polymerase, RpoA/D/Rpb3-type
InterPro IPR036603 RNA polymerase, RBP11-like subunit
InterPro IPR036643 DNA-directed RNA polymerase, insert domain superfamily
PFam PF01000 DNA-directed RNA polymerase, insert domain
PFam PF01193 DNA-directed RNA polymerase, RpoA/D/Rpb3-type
Superfamily SSF55257 RNA polymerase, RBP11-like subunit
Superfamily SSF56553 DNA-directed RNA polymerase, insert domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
30379851 Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. Briquet S, Ourimi A, ..., Vaquero C 10.1371/journal.pone.0205596
29444801 Widespread and precise reprogramming of yeast protein-genome interactions inresponse to heat shock Vinayachandran V, Reja R, ..., Pugh BF 10.1101/gr.226761.117