Product Description: OTU domain-containing protein, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.22.- (Cysteine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0004843 | thiol-dependent deubiquitinase |
| Process | GO:0016579 | protein deubiquitination |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.00 | 1.00 |
| Trophozoite | not expressed | 0.01 | 0.99 |
| Schizont | expressed | 0.00 | 1.00 |
| Gametocyte | not expressed | 0.08 | 0.95 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0923100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.999 | MFS: -1.642 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0824000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0824000)
Modification: Disrupted | RMgm-2405
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2S5
"Best" AlphaFill ligand hit: 4LJ (1.7.6 3-bromanylpropan-1-amine, Local RMSD=0.49) with 3PSE (Global RMSD=4.13)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.- | Ex: staphylococcal cysteine proteinase | NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 12 | 4 | 12 |
| disruptive | 4 | 15 | 5 | 30 |
| missense | 4 | 15 | 5 | 29 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 20 | 9 | 10 |
| disruptive | 7 | 39 | 25 | 49 |
| missense | 7 | 31 | 23 | 33 |
PlasmoDB Total SNPs: 9
Non-coding: 0 | Synonymous: 5 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 222 | Molecular Weight (kDa): 27.069
UniProt ID(s): Q8I2S5
PDB ID(s): None
Isoelectric Point: 9.36
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003323 | OTU domain |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | PF02338 | OTU domain |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 28423214 | Plasmodium falciparum O TU-like cysteine protease (Pf OTU) is essential forapicoplast homeostasis and associates with noncanonical role of Atg8 | Datta G, Hossain ME, ..., Mohmmed A | 10.1111/cmi.12748 |