Product Description: nitric oxide synthase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.14.13.39 (Nitric-oxide synthase (NADPH))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0010181 | FMN binding |
| Function | GO:0003958 | NADPH-hemoprotein reductase activity |
| Function | GO:0050660 | flavin adenine dinucleotide binding |
| Function | GO:0016491 | oxidoreductase activity |
| Function | GO:0016709 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.34 | 0.83 |
| Trophozoite | expressed | 0.36 | 0.80 |
| Schizont | expressed | 0.04 | 0.98 |
| Gametocyte | expressed | 0.27 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0923200
Resistome Missense Mutations: R35K
Resistome Compounds with Missense Mutations: MMV982556
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.156 | MFS: -3.209 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0824100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H553
"Best" AlphaFill ligand hit: NAP (nadp nicotinamide-adenine-dinucleotide phosphate, Local RMSD=0.06) with 6NJR (Global RMSD=2.92)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.14.13.39 | nitric-oxide synthase (NADPH) | CONOPINEDTAEGTACO/O2cyanideA-23187NXN-188agmatineimidazoleAR-R17477... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P16435 | NADPH--cytochrome P450 reductase | Homo sapiens | HOGENOM | CHEMBL4094677CHEMBL4086536 |
Ortholog Group (OrthoMCL): OG6_100886
Most Similar Human Ortholog: E7EMD0
TM-align score: 0.84 | RMSD: 2.81
Seq Identity: 0.27 | Length: 391 / 780
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000127948 | cytochrome p450 oxidoreductase |
Protein Length: 780 | Molecular Weight (kDa): 92.181
UniProt ID(s): C0H553
PDB ID(s): None
Isoelectric Point: 9.34
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001094 | Flavodoxin-like |
| InterPro | IPR001433 | Oxidoreductase FAD/NAD(P)-binding |
| InterPro | IPR003097 | Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding |
| InterPro | IPR008254 | Flavodoxin/nitric oxide synthase |
| InterPro | IPR017938 | Riboflavin synthase-like beta-barrel |
| InterPro | IPR029039 | Flavoprotein-like superfamily |
| InterPro | IPR039261 | Ferredoxin-NADP reductase (FNR), nucleotide-binding domain |
| PFam | PF00175 | Oxidoreductase FAD/NAD(P)-binding |
| PFam | PF00258 | Flavodoxin/nitric oxide synthase |
| PFam | PF00667 | Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding |
| Superfamily | SSF52218 | Flavoprotein-like superfamily |
| Superfamily | SSF52343 | Ferredoxin-NADP reductase (FNR), nucleotide-binding domain |
| Superfamily | SSF63380 | Riboflavin synthase-like beta-barrel |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 38 | 14 | 46 |
| disruptive | 12 | 50 | 32 | 92 |
| missense | 10 | 48 | 31 | 88 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 68 | 27 | 44 |
| disruptive | 19 | 117 | 73 | 169 |
| missense | 16 | 111 | 64 | 131 |
PlasmoDB Total SNPs: 156
Non-coding: 107 | Synonymous: 33 | Nonsynonymous: 16 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |