About Gene List

PF3D7_0923500

Genome location: Pf3D7_09_v3:954,558..958,587(+)

Genome classification: Core

Function and Localization

Product Description: cyclin-dependent kinases regulatory subunit, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.5.2 (Small monomeric GTPase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.02 0.99
Trophozoite not expressed 0.10 0.93
Schizont expressed 0.10 0.92
Gametocyte not expressed 0.14 0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0923500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.147 | MFS: -2.901 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0824400)

  • Relative Growth Rate: 0.96 ± 0.16
  • Confidence: 5.08

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0824400)

Modification: Tagged | RMgm-4850

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2S1

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.6.5.2 small monomeric GTPase GTPGDPEDTAPlexAstatinCCG-977Rap1GapNSC23766CCG-1423Rap1GAP1GTPgammaSCalmodulin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100899

Most Similar Human Ortholog: P33552

TM-align score: 0.82 | RMSD: 2.18

Seq Identity: 0.25 | Length: 77 / 357

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000123975 CDC28 protein kinase regulatory subunit 2
ENSG00000173207 CDC28 protein kinase regulatory subunit 1B

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 2 9 6 11
disruptive 8 33 11 28
missense 4 22 7 18

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 4 19 8 10
disruptive 20 52 21 65
missense 10 33 13 32

PlasmoDB Total SNPs: 323

Non-coding: 214 | Synonymous: 92 | Nonsynonymous: 17 | Stop Codon: 0

Protein Information

Protein Length: 357 | Molecular Weight (kDa): 41.873

UniProt ID(s): Q8I2S1

PDB ID(s): None

Isoelectric Point: 10.31

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000789 Cyclin-dependent kinase, regulatory subunit
InterPro IPR036858 Cyclin-dependent kinase, regulatory subunit superfamily
PFam PF01111 Cyclin-dependent kinase, regulatory subunit
Superfamily SSF55637 Cyclin-dependent kinase, regulatory subunit superfamily

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104