About Gene List

PF3D7_0923600 (LipL2)

Genome location: Pf3D7_09_v3:958,734..960,089(+)

Genome classification: Core

Function and Localization

Product Description: lipoate-protein ligase 2

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.63 (Transferred entry: 6.3.1.20);6.3.1.20 (Lipoate--protein ligase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Component GO:0005739 mitochondrion
Function GO:0016979 lipoate-protein ligase activity
Function GO:0017118 lipoyltransferase activity
Process GO:0006085 acetyl-CoA biosynthetic process
Process GO:0009249 protein lipoylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.03 0.98
Trophozoite possibly expressed 0.17 0.89
Schizont not expressed 0.09 0.93
Gametocyte possibly expressed 0.14 0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0923600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.128 | MFS: -3.099 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0824500)

  • Relative Growth Rate: 0.44 ± 0.06
  • Confidence: 6.90

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0824500)

Modification: Disrupted | RMgm-2410

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2S0

"Best" AlphaFill ligand hit: LPA (lipoic acid, Local RMSD=0.45) with 8CRI (Global RMSD=15.55)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.7.7.63 lipoate-protein ligase No BRENDA inhibitors
6.3.1.20 lipoate-protein ligase NSC68342NSC96317NSC164080NSC118483NSC118476NSC118473NSC227190NSC245342Decanoic acid8-thiooctanoate8-bromooctanoate6-seleno-octanoate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_111431

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 3 19 6 14
disruptive 1 26 15 45
missense 0 25 15 35

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 8 40 16 28
disruptive 6 78 36 151
missense 5 59 24 72

PlasmoDB Total SNPs: 63

Non-coding: 29 | Synonymous: 22 | Nonsynonymous: 11 | Stop Codon: 1

Protein Information

Protein Length: 384 | Molecular Weight (kDa): 46.089

UniProt ID(s): Q19X43, Q8I2S0

PDB ID(s): None

Isoelectric Point: 9.31

Protein Domain Annotations:

Source Family ID Description
InterPro N/A N/A
PFam N/A N/A
Superfamily SSF55681 N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
17244193 Scavenging of the cofactor lipoate is essential for the survival of the malariaparasite Plasmodium falciparum. Allary M, Lu JZ, Zhu L, Prigge ST 10.1111/j.1365-2958.2007.05592.x
18069893 Apicoplast lipoic acid protein ligase B is not essential for Plasmodiumfalciparum. Gunther S, Wallace L, ..., Muller S 10.1371/journal.ppat.0030189
28836704 A novel lipoate attachment enzyme is shared by Plasmodium and Chlamydia species. Afanador GA, Guerra AJ, ..., Prigge ST 10.1111/mmi.13776
30212465 Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. Boucher MJ, Ghosh S, ..., Yeh E 10.1371/journal.pbio.2005895
37068227 The mitochondrion of Plasmodium falciparum is required for cellular acetyl-CoAmetabolism and protein acetylation Nair SC, Munro JT, ..., Prigge ST 10.1073/pnas.2210929120
25116855 Redox-dependent lipoylation of mitochondrial proteins in Plasmodium falciparum Afanador GA, Matthews KA, ..., Prigge ST 10.1111/mmi.12753