Product Description: lipoate-protein ligase 2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.63 (Transferred entry: 6.3.1.20);6.3.1.20 (Lipoate--protein ligase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0020011 | apicoplast |
Component | GO:0005739 | mitochondrion |
Function | GO:0016979 | lipoate-protein ligase activity |
Function | GO:0017118 | lipoyltransferase activity |
Process | GO:0006085 | acetyl-CoA biosynthetic process |
Process | GO:0009249 | protein lipoylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | not expressed | N/A | N/A |
Ring | not expressed | 0.03 | 0.98 |
Trophozoite | possibly expressed | 0.17 | 0.89 |
Schizont | not expressed | 0.09 | 0.93 |
Gametocyte | possibly expressed | 0.14 | 0.92 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0923600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.128 | MFS: -3.099 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0824500)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0824500)
Modification: Disrupted | RMgm-2410
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2S0
"Best" AlphaFill ligand hit: LPA (lipoic acid, Local RMSD=0.45) with 8CRI (Global RMSD=15.55)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 3 | 19 | 6 | 14 |
disruptive | 1 | 26 | 15 | 45 |
missense | 0 | 25 | 15 | 35 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 8 | 40 | 16 | 28 |
disruptive | 6 | 78 | 36 | 151 |
missense | 5 | 59 | 24 | 72 |
PlasmoDB Total SNPs: 63
Non-coding: 29 | Synonymous: 22 | Nonsynonymous: 11 | Stop Codon: 1
PMID | Title | Authors | DOI/Link |
---|---|---|---|
12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
17244193 | Scavenging of the cofactor lipoate is essential for the survival of the malariaparasite Plasmodium falciparum. | Allary M, Lu JZ, Zhu L, Prigge ST | 10.1111/j.1365-2958.2007.05592.x |
18069893 | Apicoplast lipoic acid protein ligase B is not essential for Plasmodiumfalciparum. | Gunther S, Wallace L, ..., Muller S | 10.1371/journal.ppat.0030189 |
28836704 | A novel lipoate attachment enzyme is shared by Plasmodium and Chlamydia species. | Afanador GA, Guerra AJ, ..., Prigge ST | 10.1111/mmi.13776 |
30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
37068227 | The mitochondrion of Plasmodium falciparum is required for cellular acetyl-CoAmetabolism and protein acetylation | Nair SC, Munro JT, ..., Prigge ST | 10.1073/pnas.2210929120 |
25116855 | Redox-dependent lipoylation of mitochondrial proteins in Plasmodium falciparum | Afanador GA, Matthews KA, ..., Prigge ST | 10.1111/mmi.12753 |