PF3D7_0924000

Genome location: Pf3D7_09_v3:971,634..977,256(+)

Genome classification: Core

Function and Localization

Product Description: patatin-like phospholipase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: 3.1.1.3 (Triacylglycerol lipase);3.1.1.4 (Phospholipase A(2))

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.41	0.42
Trophozoite	expressed	0.95	0.51
Schizont	expressed	1.84	0.27
Gametocyte	possibly expressed	1.53	0.30

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0924000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.942 | MFS: -2.847 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0824900)

Relative Growth Rate: 0.69 ± 0.32
Confidence: 3.67

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0824900)

Modification: Disrupted | RMgm-2412

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2R7

"Best" AlphaFill ligand hit: PGO (s-1,2-propanediol, Local RMSD=1.72) with 4KS6 (Global RMSD=15.19)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.1.3	triacylglycerol lipase	BRENDA SOAP query unsuccessful
3.1.1.4	phospholipase A2	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102894

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	14	72	45	75
disruptive	42	189	104	186
missense	32	137	88	145

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	25	135	59	87
disruptive	103	341	126	306
missense	60	251	99	220

PlasmoDB Total SNPs: 357

Non-coding: 215 | Synonymous: 68 | Nonsynonymous: 74 | Stop Codon: 0

Protein Information

Protein Length: 1292 | Molecular Weight (kDa): 151.451

UniProt ID(s): Q8I2R7

PDB ID(s): None

Isoelectric Point: 7.34

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002641	Patatin-like phospholipase domain
InterPro	IPR016035	Acyl transferase/acyl hydrolase/lysophospholipase
InterPro	IPR021771	Triacylglycerol lipase
PFam	PF01734	Patatin-like phospholipase domain
PFam	PF11815	Triacylglycerol lipase
Superfamily	SSF52151	Acyl transferase/acyl hydrolase/lysophospholipase