Product Description: thiamine pyrophosphokinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.6.2 (Thiamine diphosphokinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004788 | thiamine diphosphokinase activity |
| Process | GO:0009229 | thiamine diphosphate biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.13 | 0.93 |
| Trophozoite | expressed | 0.42 | 0.75 |
| Schizont | possibly expressed | 0.32 | 0.80 |
| Gametocyte | expressed | 0.33 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0924300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.141 | MFS: -2.895 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0825200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0825200)
Modification: Disrupted | RMgm-2413
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H556
"Best" AlphaFill ligand hit: VIB (3-(4-amino-2-methyl-pyrimidin-5-ylmethyl)-5-(2-hydroxy-ethyl)-4-methyl-thiazol-3-ium, Local RMSD=0.19) with 3LM8 (Global RMSD=7.72)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.6.2 | thiamine diphosphokinase | AMPNEMKCNNaFPCMBALT-711molybdatediphosphateoxythiaminepyrithiamineiodoacetamideButylthiamine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101973
Most Similar Human Ortholog: Q9H3S4
TM-align score: 0.91 | RMSD: 1.80
Seq Identity: 0.34 | Length: 236 / 400
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000196511 | thiamin pyrophosphokinase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 28 | 7 | 23 |
| disruptive | 2 | 49 | 26 | 71 |
| missense | 2 | 48 | 26 | 65 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 37 | 16 | 27 |
| disruptive | 8 | 103 | 51 | 113 |
| missense | 6 | 95 | 46 | 84 |
PlasmoDB Total SNPs: 34
Non-coding: 6 | Synonymous: 16 | Nonsynonymous: 12 | Stop Codon: 0
Protein Length: 400 | Molecular Weight (kDa): 46.709
PDB ID(s): None
Isoelectric Point: 8.87
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006282 | Thiamin pyrophosphokinase |
| InterPro | IPR007371 | Thiamin pyrophosphokinase, catalytic domain |
| InterPro | IPR007373 | Thiamin pyrophosphokinase, thiamin-binding domain |
| InterPro | IPR036371 | Thiamin pyrophosphokinase, thiamin-binding domain superfamily |
| InterPro | IPR036759 | Thiamin pyrophosphokinase, catalytic domain superfamily |
| PFam | PF04263 | Thiamin pyrophosphokinase, catalytic domain |
| PFam | PF04265 | Thiamin pyrophosphokinase, thiamin-binding domain |
| Superfamily | SSF63862 | Thiamin pyrophosphokinase, thiamin-binding domain superfamily |
| Superfamily | SSF63999 | Thiamin pyrophosphokinase, catalytic domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 17132104 | The human malaria parasite Plasmodium falciparum expresses an atypicalN-terminally extended pyrophosphokinase with specificity for thiamine. | Eschbach ML, Muller IB, ..., Wrenger C | 10.1515/BC.2006.197 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |