Genome location: Pf3D7_09_v3:1,017,310..1,018,970(+)
Genome classification: Core
Product Description: ribosomal RNA-processing protein 8, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.- (Methyltransferases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005730 | nucleolus |
| Component | GO:0005634 | nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.76 | 0.65 |
| Trophozoite | expressed | 0.47 | 0.74 |
| Schizont | not expressed | 0.09 | 0.95 |
| Gametocyte | not expressed | 0.96 | 0.50 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0925200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.18 | MFS: -2.846 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2Q6
"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.51) with 2ZFU (Global RMSD=2.07)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.- | Ex: homocysteine S-methyltransferase | EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102474
Most Similar Human Ortholog: E9PPP6
TM-align score: 0.72 | RMSD: 2.40
Seq Identity: 0.33 | Length: 117 / 413
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000132275 | ribosomal RNA processing 8 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 15 | 8 | 27 |
| disruptive | 11 | 69 | 27 | 78 |
| missense | 6 | 59 | 21 | 60 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 36 | 20 | 25 |
| disruptive | 46 | 127 | 68 | 144 |
| missense | 18 | 97 | 48 | 95 |
PlasmoDB Total SNPs: 114
Non-coding: 66 | Synonymous: 33 | Nonsynonymous: 15 | Stop Codon: 0
Protein Length: 413 | Molecular Weight (kDa): 49.196
UniProt ID(s): Q8I2Q6
PDB ID(s): None
Isoelectric Point: 10.44
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007823 | Ribosomal RNA processing protein 8 |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF05148 | Ribosomal RNA processing protein 8 |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30093689 | A single N(1)-methyladenosine on the large ribosomal subunit rRNA impacts locallyits structure and the translation of key metabolic enzymes | Sharma S, Hartmann JD, ..., Entian KD | 10.1038/s41598-018-30383-z |
| 29567958 | PHA-4/FoxA senses nucleolar stress to regulate lipid accumulation in Caenorhabditis elegans | Wu J, Jiang X, ..., Liang B | 10.1038/s41467-018-03531-2 |
| 23558746 | Identification of a novel methyltransferase, Bmt2, responsible for theN-1-methyl-adenosine base modification of 25S rRNA in Saccharomyces cerevisiae | Sharma S, Watzinger P, Kotter P, Entian KD | 10.1093/nar/gkt195 |