Genome location: Pf3D7_09_v3:1,019,164..1,020,903(-)
Genome classification: Core
Product Description: proline--tRNA ligase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.15 (Proline--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004827 | proline-tRNA ligase activity |
| Process | GO:0006433 | prolyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.07 | 0.96 |
| Trophozoite | possibly expressed | 0.58 | 0.66 |
| Schizont | expressed | 0.37 | 0.77 |
| Gametocyte | possibly expressed | 0.31 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0925300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.194 | MFS: -3.341 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2Q5
"Best" AlphaFill ligand hit: BU1 (1,4-butanediol, Local RMSD=0.28) with 7F97 (Global RMSD=4.55)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.15 | proline-tRNA ligase | KClNH4ClPyrroleL-prolineglyburideL-cysteinecysteamine3-pyrrolinePyrrolidinefebrifuginehalofuginoneTCMDC-124506... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P78600 | Proline--tRNA ligase, cytoplasmic | Candida albicans | OrthoMCL BLAST | CHEMBL357572CHEMBL151769CHEMBL150306CHEMBL153238... |
Ortholog Group (OrthoMCL): OG6_103003
Most Similar Human Ortholog: Q7L3T8
TM-align score: 0.77 | RMSD: 2.59
Seq Identity: 0.24 | Length: 392 / 579
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000162396 | prolyl-tRNA synthetase 2, mitochondrial |
Protein Length: 579 | Molecular Weight (kDa): 68.466
UniProt ID(s): Q8I2Q5
PDB ID(s): None
Isoelectric Point: 9.78
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002314 | Aminoacyl-tRNA synthetase, class II (G/ P/ S/T) |
| PFam | PF00587 | Aminoacyl-tRNA synthetase, class II (G/ P/ S/T) |
| Superfamily | SSF52954 | N/A |
| Superfamily | SSF55681 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 24 | 17 | 40 |
| disruptive | 8 | 38 | 19 | 69 |
| missense | 8 | 38 | 17 | 63 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 56 | 24 | 41 |
| disruptive | 24 | 110 | 65 | 118 |
| missense | 16 | 86 | 48 | 68 |
PlasmoDB Total SNPs: 47
Non-coding: 0 | Synonymous: 35 | Nonsynonymous: 9 | Stop Codon: 3
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25047712 | Structural and functional analysis of the anti-malarial drug target prolyl-tRNAsynthetase. | Jain V, Kikuchi H, ..., Yogavel M | 10.1007/s10969-014-9186-x |
| 25995223 | The cytoplasmic prolyl-tRNA synthetase of the malaria parasite is a dual-stagetarget of febrifugine and its analogs. | Herman JD, Pepper LR, ..., Mazitschek R | 10.1126/scitranslmed.aaa3575 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |