PF3D7_0925400

Genome location: Pf3D7_09_v3:1,021,426..1,023,671(-)

Genome classification: Core

Function and Localization

Product Description: protein phosphatase-beta

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.02	0.99
Trophozoite	expressed	0.10	0.94
Schizont	expressed	0.05	0.97
Gametocyte	expressed	0.30	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0925400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -2.96 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0826200)

Relative Growth Rate: 0.11 ± 0.09
Confidence: 6.14

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0826200)

Modification: Tagged | RMgm-1066

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2Q4

"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.26) with 6GHM (Global RMSD=1.31)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.3.16	protein-serine/threonine phosphatase	ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100547

Most Similar Human Ortholog: E5RFI3

TM-align score: 0.99 | RMSD: 0.50

Seq Identity: 0.66 | Length: 125 / 466

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000104695	protein phosphatase 2 catalytic subunit beta
ENSG00000113575	protein phosphatase 2 catalytic subunit alpha

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	17	11	36
disruptive	7	22	12	54
missense	7	21	12	46

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	44	14	31
disruptive	17	55	39	99
missense	13	43	36	71

PlasmoDB Total SNPs: 146

Non-coding: 123 | Synonymous: 13 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 466 | Molecular Weight (kDa): 53.912

UniProt ID(s): O15920, Q8I2Q4

PDB ID(s): None

Isoelectric Point: 4.72

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004843	Calcineurin-like phosphoesterase domain, ApaH type
InterPro	IPR006186	Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase
PFam	PF00149	Calcineurin-like phosphoesterase domain, ApaH type
Superfamily	SSF56300	N/A