About Gene List

PF3D7_0925900 (LCN)

Genome location: Pf3D7_09_v3:1,039,462..1,042,118(+)

Genome classification: Core

Function and Localization

Product Description: lipocalin

SignalP Peptide: MIKKAVSLFFIFFLFTIIRA

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005829 cytosol
Component GO:0020020 food vacuole
Component GO:0016020 membrane
Component GO:0020003 symbiont-containing vacuole
Process GO:0110148 biomineralization
Process GO:0006979 response to oxidative stress

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 1.11 0.50
Trophozoite expressed 0.98 0.50
Schizont expressed 0.16 0.91
Gametocyte expressed 0.44 0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0925900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.219 | MFS: -3.309 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0826700)

Modification: Mutated | RMgm-4853

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2Q0

"Best" AlphaFill ligand hit: HXA (docosa-4,7,10,13,16,19-hexaenoic acid, Local RMSD=1.25) with 5Y5C (Global RMSD=14.62)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532555

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 1 9 8 13
disruptive 2 2 1 10
missense 2 2 1 9

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 1 21 12 21
disruptive 3 12 20 52
missense 3 5 16 38

PlasmoDB Total SNPs: 143

Non-coding: 133 | Synonymous: 9 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 217 | Molecular Weight (kDa): 24.698

UniProt ID(s): Q8I2Q0

PDB ID(s): 6TLB

Isoelectric Point: 5.3

Protein Domain Annotations:

Source Family ID Description
InterPro IPR012674 Calycin
PFam N/A N/A
Superfamily SSF50814 Calycin

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
21136929 Food vacuole proteome of the malarial parasite Plasmodium falciparum. Lamarque M, Tastet C, ..., Dubremetz JF 10.1002/prca.200700112
30232409 An in silico down-scaling approach uncovers novel constituents of thePlasmodium-containing vacuole. Matz JM, Matuschewski K 10.1038/s41598-018-32471-6
32579913 Structure-Based Identification and Functional Characterization of a Lipocalin inthe Malaria Parasite Plasmodium falciparum. Burda PC, Crosskey T, ..., Gilberger TW 10.1016/j.celrep.2020.107817
32601225 A lipocalin mediates unidirectional heme biomineralization in malaria parasites. Matz JM, Drepper B, ..., Blackman MJ 10.1073/pnas.2001153117