PF3D7_0926000

Genome location: Pf3D7_09_v3:1,043,374..1,048,185(+)

Genome classification: Core

Function and Localization

Product Description: protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0009931	calcium-dependent protein serine/threonine kinase activity
Function	GO:0005516	calmodulin binding
Process	GO:0035556	intracellular signal transduction
Process	GO:0018105	peptidyl-serine phosphorylation
Process	GO:0046777	protein autophosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.34	0.83
Trophozoite	expressed	0.53	0.70
Schizont	expressed	0.11	0.94
Gametocyte	expressed	0.48	0.74

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0926000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.657 | #Insertions: 4

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2P9

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532978

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	60	38	92
disruptive	60	215	122	315
missense	36	168	102	269

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	19	132	49	107
disruptive	105	476	220	416
missense	42	377	183	297

PlasmoDB Total SNPs: 299

Non-coding: 129 | Synonymous: 105 | Nonsynonymous: 63 | Stop Codon: 2

Protein Information

Protein Length: 1277 | Molecular Weight (kDa): 153.345

UniProt ID(s): Q8I2P9

PDB ID(s): None

Isoelectric Point: 9.29

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain
InterPro	IPR003409	MORN motif
InterPro	IPR011009	Protein kinase-like domain superfamily
InterPro	IPR013761	Sterile alpha motif/pointed domain superfamily
PFam	PF00069	Protein kinase domain
PFam	PF02493	MORN motif
PFam	PF07714	Serine-threonine/tyrosine-protein kinase, catalytic domain
Superfamily	SSF47769	Sterile alpha motif/pointed domain superfamily
Superfamily	SSF56112	Protein kinase-like domain superfamily
Superfamily	SSF82185