PF3D7_0926300

Genome location: Pf3D7_09_v3:1,064,388..1,070,885(+)

Genome classification: Core

Function and Localization

Product Description: protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase);2.7.11.17 (Calcium/calmodulin-dependent protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:1903561	extracellular vesicle
Function	GO:0004674	protein serine/threonine kinase activity
Process	GO:0046777	protein autophosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.19	0.90
Trophozoite	expressed	0.24	0.86
Schizont	expressed	0.12	0.93
Gametocyte	expressed	1.06	0.48

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0926300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.124 | MFS: -2.828 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0827100)

Modification: Disrupted | RMgm-2422

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H563

"Best" AlphaFill ligand hit: HVK (pyridin-2-amine, Local RMSD=0.10) with 6QA1 (Global RMSD=5.32)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...
2.7.11.17	Ca2+/calmodulin-dependent protein kinase	NOADPGTPW13AIPEDTAEGTADTNBKN93KN62KN-93KN-62...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532312

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	49	31	72
disruptive	21	101	43	161
missense	16	94	39	139

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	105	36	73
disruptive	52	195	107	244
missense	29	174	92	185

PlasmoDB Total SNPs: 519

Non-coding: 414 | Synonymous: 67 | Nonsynonymous: 38 | Stop Codon: 0

Protein Information

Protein Length: 1179 | Molecular Weight (kDa): 141.14

UniProt ID(s): C0H563

PDB ID(s): None

Isoelectric Point: 8.9

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR008271	Serine/threonine-protein kinase, active site
InterPro	IPR011009	Protein kinase-like domain superfamily
PFam	PF14381	N/A
Superfamily	SSF56112	Protein kinase-like domain superfamily

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
33608523	20S proteasomes secreted by the malaria parasite promote its growth.	Dekel E, Yaffe D, ..., Regev-Rudzki N	10.1038/s41467-021-21344-8