Product Description: fumarate hydratase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.2.1.2 (Fumarate hydratase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004333 | fumarate hydratase activity |
| Process | GO:0006106 | fumarate metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.37 | 0.77 |
| Schizont | expressed | 0.64 | 0.64 |
| Gametocyte | expressed | 0.33 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0927300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.127 | MFS: -2.952 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0828100)
Modification: Disrupted | RMgm-4427
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2N6
"Best" AlphaFill ligand hit: LMR (l-malate, Local RMSD=0.01) with 6UQM (Global RMSD=1.06)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.2.1.2 | fumarate hydratase | ATPADPAMPGTPCTPUTPNEMSDSEDTAZnCl2NH4ClCuCl2... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 30 | 19 | 28 |
| disruptive | 2 | 46 | 25 | 64 |
| missense | 2 | 44 | 25 | 57 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 57 | 22 | 50 |
| disruptive | 7 | 92 | 49 | 129 |
| missense | 6 | 80 | 41 | 100 |
PlasmoDB Total SNPs: 104
Non-coding: 86 | Synonymous: 11 | Nonsynonymous: 6 | Stop Codon: 1
Protein Length: 681 | Molecular Weight (kDa): 78.396
UniProt ID(s): Q8I2N6
PDB ID(s): None
Isoelectric Point: 9.39
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004646 | Fe-S hydro-lyase, tartrate dehydratase alpha-type, catalytic domain |
| InterPro | IPR004647 | Fe-S hydro-lyase, tartrate dehydratase beta-type, catalytic domain |
| InterPro | IPR036660 | Fe-S hydro-lyase, tartrate dehydratase beta-type, catalytic domain superfamily |
| PFam | PF05681 | Fe-S hydro-lyase, tartrate dehydratase alpha-type, catalytic domain |
| PFam | PF05683 | Fe-S hydro-lyase, tartrate dehydratase beta-type, catalytic domain |
| Superfamily | SSF117457 | Fe-S hydro-lyase, tartrate dehydratase beta-type, catalytic domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 29449371 | Biochemical characterization and essentiality of Plasmodium fumarate hydratase. | Jayaraman V, Suryavanshi A, ..., Balaram H | 10.1074/jbc.M117.816298 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |