Genome location: Pf3D7_09_v3:1,126,803..1,128,396(-)
Genome classification: Core
Product Description: serine/threonine protein phosphatase 4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.11 | 0.93 |
| Schizont | expressed | 0.11 | 0.92 |
| Gametocyte | expressed | 0.11 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0927700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.131 | MFS: -2.938 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0828500)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0828500)
Modification: Disrupted | RMgm-2428
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2N2
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.18) with 6GHM (Global RMSD=1.05)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P62136 | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit | Homo sapiens | HOGENOM, OrthoDB | MLS001241052 |
| P36873 | Serine/threonine-protein phosphatase PP1-gamma catalytic subunit | Homo sapiens | OrthoDB | CHEMBL200117CHEMBL202197okadaic acidokadaic acid... |
| P67774 | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform | Bos taurus | OrthoDB | CHEMBL318253CHEMBL421377CHEMBL421377CHEMBL318253... |
| P67775 | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform | Homo sapiens | OrthoDB | okadaic acid analogueokadaic acid |
Ortholog Group (OrthoMCL): OG6_101963
Most Similar Human Ortholog: H3BV22
TM-align score: 0.97 | RMSD: 1.19
Seq Identity: 0.58 | Length: 201 / 312
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000149923 | protein phosphatase 4 catalytic subunit |
Protein Length: 312 | Molecular Weight (kDa): 36.387
UniProt ID(s): Q8I2N2
PDB ID(s): None
Isoelectric Point: 7.16
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004843 | Calcineurin-like phosphoesterase domain, ApaH type |
| InterPro | IPR006186 | Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase |
| PFam | PF00149 | Calcineurin-like phosphoesterase domain, ApaH type |
| Superfamily | SSF56300 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 10 | 8 | 11 |
| disruptive | 3 | 11 | 9 | 18 |
| missense | 3 | 11 | 8 | 16 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 31 | 9 | 20 |
| disruptive | 8 | 32 | 19 | 72 |
| missense | 6 | 23 | 14 | 43 |
PlasmoDB Total SNPs: 49
Non-coding: 41 | Synonymous: 6 | Nonsynonymous: 2 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |