PF3D7_0928800

Genome location: Pf3D7_09_v3:1,155,523..1,159,304(+)

Genome classification: Core

Function and Localization

Product Description: serine/threonine protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.04	0.98
Trophozoite	expressed	0.31	0.81
Schizont	expressed	0.17	0.90
Gametocyte	expressed	0.42	0.76

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0928800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.175 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0829600)

Relative Growth Rate: 1.00 ± 0.05
Confidence: 7.35

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0829600)

Modification: Disrupted | RMgm-2437

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I0W2

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=3.76) with 3Q5I (Global RMSD=4.57)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532355

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	46	25	48
disruptive	39	138	64	163
missense	18	97	48	122

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	16	87	31	50
disruptive	72	283	98	230
missense	30	198	71	150

PlasmoDB Total SNPs: 326

Non-coding: 156 | Synonymous: 83 | Nonsynonymous: 87 | Stop Codon: 0

Protein Information

Protein Length: 820 | Molecular Weight (kDa): 99.169

UniProt ID(s): Q8I0W2

PDB ID(s): None

Isoelectric Point: 10.12

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR008271	Serine/threonine-protein kinase, active site
InterPro	IPR011009	Protein kinase-like domain superfamily
PFam	PF00069	Protein kinase domain
Superfamily	SSF56112	Protein kinase-like domain superfamily