Product Description: guanylate kinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.4.8 (Guanylate kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Function | GO:0004385 | guanylate kinase activity |
| Process | GO:0006186 | dGDP phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.09 | 0.95 |
| Trophozoite | possibly expressed | 0.23 | 0.86 |
| Schizont | not expressed | 0.03 | 0.98 |
| Gametocyte | not expressed | 0.12 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0928900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.228 | MFS: -3.401 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0829700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0829700)
Modification: Disrupted | RMgm-2438
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2M1
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.10) with 8EGL (Global RMSD=1.96)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.4.8 | guanylate kinase | ATPAMPGTPGDPCMPUMPGMPEDTAPMSFdCMPdAMPdGMP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100603
Most Similar Human Ortholog: Q16774
TM-align score: 0.91 | RMSD: 1.69
Seq Identity: 0.36 | Length: 193 / 199
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000143774 | guanylate kinase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 9 | 5 | 16 |
| disruptive | 1 | 0 | 2 | 14 |
| missense | 1 | 0 | 2 | 13 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 22 | 8 | 13 |
| disruptive | 1 | 13 | 17 | 37 |
| missense | 1 | 7 | 14 | 28 |
PlasmoDB Total SNPs: 117
Non-coding: 104 | Synonymous: 9 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 199 | Molecular Weight (kDa): 23.515
UniProt ID(s): Q8I2M1
PDB ID(s): 1Z6G
Isoelectric Point: 8.57
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR008145 | Guanylate kinase/L-type calcium channel beta subunit |
| InterPro | IPR017665 | Guanylate kinase |
| InterPro | IPR020590 | Guanylate kinase, conserved site |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00625 | Guanylate kinase/L-type calcium channel beta subunit |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 18374996 | Molecular cloning, expression, characterization and mutation of Plasmodiumfalciparum guanylate kinase. | Kandeel M, Nakanishi M, ..., Kitade Y | 10.1016/j.molbiopara.2008.02.004 |