Genome location: Pf3D7_09_v3:1,173,579..1,181,380(+)
Genome classification: Core
Product Description: high molecular weight rhoptry protein 2
SignalP Peptide: MIKVTIFLLLSIFSFNLYG
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0030430 | host cell cytoplasm |
| Component | GO:0033644 | host cell membrane |
| Component | GO:0005634 | nucleus |
| Component | GO:0020008 | rhoptry |
| Component | GO:0020005 | symbiont-containing vacuole membrane |
| Function | GO:0003723 | RNA binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0044877 | protein-containing complex binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.24 | 0.89 |
| Trophozoite | expressed | 0.69 | 0.67 |
| Schizont | expressed | 3.25 | 0.14 |
| Gametocyte | expressed | 0.45 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0929400
Resistome Missense Mutations: P1245A
Resistome Compounds with Missense Mutations: MMV006901
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.01 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0830200)
Modification: Tagged | RMgm-4720
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H571
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.85) with 5TVK (Global RMSD=19.75)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 50 | 21 | 50 |
| disruptive | 29 | 24 | 30 | 78 |
| missense | 28 | 21 | 27 | 70 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 72 | 44 | 68 |
| disruptive | 38 | 78 | 58 | 156 |
| missense | 34 | 62 | 50 | 114 |
PlasmoDB Total SNPs: 420
Non-coding: 375 | Synonymous: 18 | Nonsynonymous: 27 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 3887156 | Isolation of a Plasmodium falciparum rhoptry protein. | Holder AA, Freeman RR, Uni S, Aikawa M | 10.1016/0166-6851(85)90057-x |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 28252383 | Plasmodium falciparum parasites deploy Rhop H2 into the host erythrocyte to obtainnutrients, grow and replicate | Counihan NA, Chisholm SA, ..., de Koning-Ward TF | 10.7554/eLife.23217 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 32900800 | Live-Cell F RET Reveals that Malaria Nutrient Channel Proteins C LAG3 and Rhop H2Remain Associated throughout Their Tortuous Trafficking | Ahmad M, Manzella-Lapeira J, ..., Desai SA | 10.1128/mBio.01354-20 |
| 33774908 | Characterisation of complexes formed by parasite proteins exported into the hostcell compartment of Plasmodium falciparum infected red blood cells. | Jonsdottir TK, Counihan NA, ..., Gilson PR | 10.1111/cmi.13332 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 35393572 | RhopH2 and RhopH3 export enables assembly of the RhopH complex on P.falciparum-infected erythrocyte membranes. | Pasternak M, Verhoef JMJ, ..., Cowman AF | 10.1038/s42003-022-03290-3 |
| 33393463 | Malaria parasites use a soluble RhopH complex for erythrocyte invasion and anintegral form for nutrient uptake | Schureck MA, Darling JE, ..., Desai SA | 10.7554/eLife.65282 |
| 21320181 | Functional analysis of epigenetic regulation of tandem RhopH1/clag genes revealsa role in Plasmodium falciparum growth | Comeaux CA, Coleman BI, ..., Duraisingh MT | 10.1111/j.1365-2958.2011.07572.x |
| 27299521 | Malaria Parasite CLAG3, a Protein Linked to Nutrient Channels, Participates inHigh Molecular Weight Membrane-Associated Complexes in the Infected Erythrocyte | Zainabadi K | 10.1371/journal.pone.0157390 |
| 18155305 | Diversity and evolution of the rhoph1/clag multigene family of Plasmodiumfalciparum | Iriko H, Kaneko O, ..., Torii M | 10.1016/j.molbiopara.2007.11.004 |
| 34446549 | Native structure of the RhopH complex, a key determinant of malaria parasitenutrient acquisition | Ho CM, Jih J, ..., Zhou ZH | 10.1073/pnas.2100514118 |
| 35096656 | Identification, Mapping, and Genetic Diversity of Novel Conserved Cross-SpeciesEpitopes of RhopH2 in Plasmodium knowlesi With Plasmodium vivax | Ahmed MA, Deshmukh GY, ..., Quan FS | 10.3389/fcimb.2021.810398 |
| 18508137 | The RhopH complex is transferred to the host cell cytoplasm following red bloodcell invasion by Plasmodium falciparum | Vincensini L, Fall G, ..., Braun Breton C | 10.1016/j.molbiopara.2008.04.002 |
| 15049814 | The Plasmodium falciparum clag9 gene encodes a rhoptry protein that istransferred to the host erythrocyte upon invasion | Ling IT, Florens L, ..., Mattei D | 10.1111/j.1365-2958.2003.03969.x |
| 32069335 | Complex nutrient channel phenotypes despite Mendelian inheritance in a Plasmodiumfalciparum genetic cross | Gupta A, Bokhari AAB, ..., Desai SA | 10.1371/journal.ppat.1008363 |
| 23539954 | Trafficking of Plasmodium falciparum chimeric rhoptry protein with Brefeldin A | Ghoneim AM | 10.14411/fp.2013.009 |
| 15953647 | Apical expression of three RhopH1/Clag proteins as components of the Plasmodiumfalciparum RhopH complex | Kaneko O, Yim Lim BY, ..., Torii M | https://pubmed.ncbi.nlm.nih.gov/15953647/ |
| 23097184 | Identification and characterization of the Plasmodium falciparum RhopH2 orthologin Plasmodium vivax | Wang B, Lu F, ..., Han ET | 10.1007/s00436-012-3170-9 |
| 12968029 | Identification of a stomatin orthologue in vacuoles induced in human erythrocytesby malaria parasites. A role for microbial raft proteins in apicomplexan vacuolebiogenesis | Hiller NL, Akompong T, ..., Haldar K | 10.1074/jbc.M307266200 |
| 28221136 | An essential dual-function complex mediates erythrocyte invasion andchannel-mediated nutrient uptake in malaria parasites | Ito D, Schureck MA, Desai SA | 10.7554/eLife.23485 |
| 31311978 | A 4-cyano-3-methylisoquinoline inhibitor of Plasmodium falciparum growth targetsthe sodium efflux pump PfATP4 | Gilson PR, Kumarasingha R, ..., Abbott BM | 10.1038/s41598-019-46500-5 |
| 17175193 | The Plasmodium falciparum RhopH2 promoter and first 24 amino acids are sufficientto target proteins to the rhoptries | Ghoneim A, Kaneko O, Tsuboi T, Torii M | 10.1016/j.parint.2006.11.001 |