Genome location: Pf3D7_09_v3:1,211,597..1,217,273(-)
Genome classification: Core
Product Description: diacylglycerol kinase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 2.7.1.107 (Diacylglycerol kinase (ATP))
Curated GO (PlasmoDB): None
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.64 | 0.69 |
| Trophozoite | possibly expressed | 0.45 | 0.74 |
| Schizont | expressed | 1.37 | 0.39 |
| Gametocyte | possibly expressed | 0.49 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0930500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.896 | MFS: -2.819 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2K9
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.107 | diacylglycerol kinase (ATP) | ATPADPUDPAMPGTPGDPCTPUTPCDPITPIDPcAMP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P23743 | Diacylglycerol kinase alpha/zeta | OrthoDB | US11584747, Example 2US11584747, Example 2US11584747, Example 4US11584747, Example 4... | |
| Q13574 | Diacylglycerol kinase zeta | OrthoDB | US11584747, Example 2US11584747, Example 2US11584747, Example 4US11584747, Example 4... | |
| P20192 | Diacylglycerol kinase alpha | OrthoDB | CHEMBL4558937CHEMBL4558937 |
Ortholog Group (OrthoMCL): OG6_106579
Most Similar Human Ortholog: P52429
TM-align score: 0.62 | RMSD: 3.51
Seq Identity: 0.26 | Length: 392 / 1360
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000153933 | diacylglycerol kinase epsilon |
Protein Length: 1360 | Molecular Weight (kDa): 160.293
UniProt ID(s): Q8I2K9
PDB ID(s): None
Isoelectric Point: 9.12
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000756 | Diacylglycerol kinase, accessory domain |
| InterPro | IPR001206 | Diacylglycerol kinase, catalytic domain |
| InterPro | IPR016064 | NAD kinase/diacylglycerol kinase-like domain superfamily |
| InterPro | IPR037607 | Diacylglycerol kinase |
| PFam | PF00609 | Diacylglycerol kinase, accessory domain |
| PFam | PF00781 | Diacylglycerol kinase, catalytic domain |
| Superfamily | SSF111331 | NAD kinase/diacylglycerol kinase-like domain superfamily |
| Superfamily | SSF57889 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 65 | 20 | 63 |
| disruptive | 58 | 150 | 84 | 214 |
| missense | 31 | 110 | 64 | 164 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 107 | 44 | 76 |
| disruptive | 110 | 315 | 166 | 317 |
| missense | 55 | 212 | 122 | 206 |
PlasmoDB Total SNPs: 170
Non-coding: 67 | Synonymous: 70 | Nonsynonymous: 33 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 32037626 | Yeast transformation stress, together with loss of Pah1, phosphatidic acidphosphatase, leads to Ty1 retrotransposon insertion into the INO4 gene | Kudo S, Shiino H, Furuta S, Tamura Y | 10.1096/fj.201901811RR |
| 32058036 | Triglyceride deficiency and diacylglycerol kinase1 activity lead to theupregulation of mevalonate pathway in yeast: A study for the development ofpotential yeast platform for improved production of triterpenoid | Ranganathan PR, Nawada N, Narayanan AK, Rao DKV | 10.1016/j.bbalip.2020.158661 |
| 35239652 | ER-PM membrane contact site regulation by yeast ORPs and membrane stresspathways | Quon E, Nenadic A, ..., Beh CT | 10.1371/journal.pgen.1010106 |
| 35661158 | Diacylglycerol kinase alleviates autophagic degradation of the endoplasmicreticulum in SPT10-deficient yeast to enhance triterpene biosynthesis | Ranganathan PR, Narayanan AK, ..., Dodaghatta Krishna VR | 10.1002/1873-3468.14418 |