Genome location: Pf3D7_09_v3:1,246,725..1,248,266(-)
Genome classification: Core
Product Description: NifU-like protein, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0051539 | 4 iron, 4 sulfur cluster binding |
| Function | GO:0005506 | iron ion binding |
| Process | GO:0016226 | iron-sulfur cluster assembly |
| Process | GO:0097428 | protein maturation by iron-sulfur cluster transfer |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.04 | 0.98 |
| Trophozoite | no data | 0.14 | 0.91 |
| Schizont | no data | 0.12 | 0.92 |
| Gametocyte | no data | 0.34 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0930900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.26 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0831700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0831700)
Modification: Disrupted | RMgm-2444
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H578
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101229
Most Similar Human Ortholog: C9J8Q1
TM-align score: 0.83 | RMSD: 2.20
Seq Identity: 0.33 | Length: 93 / 192
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000169599 | NFU1 iron-sulfur cluster scaffold |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 8 | 5 | 12 |
| disruptive | 4 | 18 | 14 | 31 |
| missense | 4 | 18 | 13 | 28 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 20 | 5 | 12 |
| disruptive | 6 | 42 | 20 | 53 |
| missense | 4 | 37 | 16 | 37 |
PlasmoDB Total SNPs: 48
Non-coding: 40 | Synonymous: 3 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 192 | Molecular Weight (kDa): 22.624
UniProt ID(s): C0H578
PDB ID(s): None
Isoelectric Point: 8.43
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001075 | NIF system FeS cluster assembly, NifU, C-terminal |
| InterPro | IPR034904 | Fe-S cluster assembly domain superfamily |
| PFam | PF01106 | NIF system FeS cluster assembly, NifU, C-terminal |
| Superfamily | SSF117916 | Fe-S cluster assembly domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23805304 | Experimental Genetics of Plasmodium berghei NFU in the Apicoplast Iron-SulfurCluster Biogenesis Pathway. | Haussig JM, Matuschewski K, Kooij TW | 10.1371/journal.pone.0067269 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |