PF3D7_0931000

Genome location: Pf3D7_09_v3:1,248,274..1,252,765(-)

Genome classification: Core

Function and Localization

Product Description: elongation factor Tu, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Component	GO:1990904	ribonucleoprotein complex
Function	GO:0003924	GTPase activity
Function	GO:0043022	ribosome binding
Function	GO:0003746	translation elongation factor activity
Process	GO:0006414	translational elongation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.31	0.84
Trophozoite	expressed	0.59	0.67
Schizont	expressed	0.22	0.86
Gametocyte	possibly expressed	0.33	0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0931000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.211 | #Insertions: 9

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0831800)

Relative Growth Rate: 1.01 ± 0.17
Confidence: 4.94

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0831800)

Modification: Disrupted | RMgm-2445

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2K5

"Best" AlphaFill ligand hit: GCP (phosphomethylphosphonic acid guanylate ester, Local RMSD=1.74) with 6U44 (Global RMSD=4.51)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533649

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	29	25	46
disruptive	27	94	61	146
missense	20	82	56	121

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	76	36	49
disruptive	61	227	113	241
missense	35	184	90	167

PlasmoDB Total SNPs: 226

Non-coding: 151 | Synonymous: 39 | Nonsynonymous: 36 | Stop Codon: 0

Protein Information

Protein Length: 911 | Molecular Weight (kDa): 107.452

UniProt ID(s): Q8I2K5

PDB ID(s): None

Isoelectric Point: 8.51

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000795	Transcription factor, GTP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00009	Transcription factor, GTP-binding domain
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108