PF3D7_0931100

Genome location: Pf3D7_09_v3:1,253,448..1,257,339(+)

Genome classification: Core

Function and Localization

Product Description: nucleolar protein Nop52, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Component	GO:0030687	preribosome, large subunit precursor
Process	GO:0006364	rRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	2.25	0.20
Trophozoite	expressed	1.03	0.48
Schizont	expressed	0.45	0.75
Gametocyte	expressed	0.90	0.53

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0931100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.525 | #Insertions: 5

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0831900)

Relative Growth Rate: 0.67 ± 0.28
Confidence: 3.94

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0831900)

Modification: Disrupted | RMgm-2446

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2K4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102179

Most Similar Human Ortholog: D6RE82

TM-align score: 0.69 | RMSD: 1.85

Seq Identity: 0.19 | Length: 37 / 825

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000160208	ribosomal RNA processing 1B
ENSG00000160214	ribosomal RNA processing 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	35	17	45
disruptive	31	121	44	128
missense	16	96	37	98

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	73	31	47
disruptive	74	265	111	249
missense	34	186	82	126

PlasmoDB Total SNPs: 243

Non-coding: 94 | Synonymous: 68 | Nonsynonymous: 80 | Stop Codon: 1

Protein Information

Protein Length: 825 | Molecular Weight (kDa): 97.929

UniProt ID(s): Q8I2K4

PDB ID(s): None

Isoelectric Point: 9.06

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR010301	Nucleolar, Nop52
PFam	PF05997	Nucleolar, Nop52
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104