PF3D7_0932100

Genome location: Pf3D7_09_v3:1,278,479..1,284,699(-)

Genome classification: Core

Function and Localization

Product Description: protein MAM3, putative

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0043231	intracellular membrane-bounded organelle

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.24	0.88
Trophozoite	expressed	0.61	0.67
Schizont	expressed	2.17	0.18
Gametocyte	expressed	0.64	0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0932100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.277 | MFS: -2.559 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0832900)

Relative Growth Rate: 0.61 ± 0.10
Confidence: 5.93

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0832900)

Modification: Disrupted | RMgm-2453

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H584

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=6.51) with 6N7E (Global RMSD=7.90)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100176

Most Similar Human Ortholog: Q6P4Q7

TM-align score: 0.44 | RMSD: 4.56

Seq Identity: 0.28 | Length: 394 / 1274

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000119946	cyclin and CBS domain divalent metal cation transport mediator 1
ENSG00000148842	cyclin and CBS domain divalent metal cation transport mediator 2
ENSG00000158158	cyclin and CBS domain divalent metal cation transport mediator 4
ENSG00000168763	cyclin and CBS domain divalent metal cation transport mediator 3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	41	44	68
disruptive	49	141	76	176
missense	19	87	54	125

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	105	54	90
disruptive	95	305	161	337
missense	33	199	104	213

PlasmoDB Total SNPs: 407

Non-coding: 260 | Synonymous: 85 | Nonsynonymous: 61 | Stop Codon: 1

Protein Information

Protein Length: 1274 | Molecular Weight (kDa): 147.392

UniProt ID(s): C0H584

PDB ID(s): None

Isoelectric Point: 9.95

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000644	CBS domain
InterPro	IPR002550	CNNM, transmembrane domain
InterPro	IPR018490	Cyclic nucleotide-binding-like
PFam	PF00571	CBS domain
PFam	PF01595	CNNM, transmembrane domain
Superfamily	SSF51206	Cyclic nucleotide-binding-like
Superfamily	SSF54631