Genome location: Pf3D7_09_v3:1,286,481..1,289,644(+)
Genome classification: Core
Product Description: profilin
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0015629 | actin cytoskeleton |
| Component | GO:0005938 | cell cortex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0003779 | actin binding |
| Function | GO:0003785 | actin monomer binding |
| Function | GO:0005543 | phospholipid binding |
| Function | GO:0070064 | proline-rich region binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0030036 | actin cytoskeleton organization |
| Process | GO:0048870 | cell motility |
| Process | GO:0060327 | cytoplasmic actin-based contraction involved in cell motility |
| Process | GO:0044409 | entry into host |
| Process | GO:0042989 | sequestering of actin monomers |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.31 | 0.87 |
| Trophozoite | expressed | 0.55 | 0.69 |
| Schizont | expressed | 1.99 | 0.19 |
| Gametocyte | expressed | 0.67 | 0.63 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0932200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.994 | MFS: -2.42 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0833000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0833000)
Modification: Mutated | RMgm-4746
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2J4
"Best" AlphaFill ligand hit: DTV ((2s,3s)-1,4-dimercaptobutane-2,3-diol, Local RMSD=0.16) with 3NEC (Global RMSD=1.98)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 1 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 207
Non-coding: 188 | Synonymous: 16 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 171 | Molecular Weight (kDa): 19.017
UniProt ID(s): Q8I2J4
PDB ID(s): None
Isoelectric Point: 3.96
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005455 | Profilin |
| InterPro | IPR016814 | Profilin, apicomplexa |
| InterPro | IPR036140 | Profilin superfamily |
| PFam | PF00235 | Profilin |
| Superfamily | SSF55770 | Profilin superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 18329618 | A malaria parasite formin regulates actin polymerization and localizes to theparasite-erythrocyte moving junction during invasion. | Baum J, Tonkin CJ, ..., Cowman AF | 10.1016/j.chom.2008.02.006 |
| 19000816 | Structural basis for parasite-specific functions of the divergent profilin ofPlasmodium falciparum. | Kursula I, Kursula P, ..., Schuler H | 10.1016/j.str.2008.09.008 |
| 23245327 | A tetracycline-repressible transactivator system to study essential genes inmalaria parasites. | Pino P, Sebastian S, ..., Soldati-Favre D | 10.1016/j.chom.2012.10.016 |
| 24080030 | Proteolytic activity of Plasmodium falciparum subtilisin-like protease 3 onparasite profilin, a multifunctional protein. | Alam A, Bhatnagar RK, ..., Chauhan VS | 10.1016/j.molbiopara.2013.09.006 |
| 28552953 | A unique profilin-actin interface is important for malaria parasite motility. | Moreau CA, Bhargav SP, ..., Frischknecht F | 10.1371/journal.ppat.1006412 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |