Genome location: Pf3D7_09_v3:1,288,799..1,291,543(-)
Genome classification: Core
Product Description: M18 aspartyl aminopeptidase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.11.21 (Aspartyl aminopeptidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.28 | 0.87 |
| Trophozoite | expressed | 0.91 | 0.52 |
| Schizont | expressed | 0.13 | 0.92 |
| Gametocyte | expressed | 0.54 | 0.69 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0932300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.476 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0833100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0833100)
Modification: Disrupted | RMgm-2455
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2J3
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.44) with 4NJQ (Global RMSD=1.78)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.11.21 | aspartyl aminopeptidase | SDSDTTE64EDTAEGTAPMSFDMSOL-GluL-AspethanolacetoneQuinine... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8I2J3 | M18 aspartyl aminopeptidase | Plasmodium falciparum (isolate 3D7) | HOGENOM, OMA, OrthoMCL, OrthoMCL BLAST | MLS000068216MLS000073496MLS000031579MLS000071546... |
| Q9ULA0 | Aspartyl aminopeptidase | Homo sapiens | OMA | CHEMBL309130CHEMBL143589CHEMBL140846CHEMBL142010 |
Ortholog Group (OrthoMCL): OG6_102047
Most Similar Human Ortholog: E7EMB6
TM-align score: 0.95 | RMSD: 1.84
Seq Identity: 0.32 | Length: 405 / 570
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000123992 | aspartyl aminopeptidase |
Protein Length: 570 | Molecular Weight (kDa): 65.635
UniProt ID(s): Q8I2J3
PDB ID(s): 4EME
Isoelectric Point: 7.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001948 | Peptidase M18 |
| PFam | PF02127 | Peptidase M18 |
| Superfamily | SSF101821 | N/A |
| Superfamily | SSF53187 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 20 | 15 | 38 |
| disruptive | 6 | 30 | 15 | 47 |
| missense | 6 | 30 | 15 | 46 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 45 | 21 | 45 |
| disruptive | 11 | 60 | 34 | 89 |
| missense | 10 | 57 | 30 | 73 |
PlasmoDB Total SNPs: 34
Non-coding: 22 | Synonymous: 8 | Nonsynonymous: 4 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 17895246 | Roles for two aminopeptidases in vacuolar hemoglobin catabolism in Plasmodiumfalciparum. | Dalal S, Klemba M | 10.1074/jbc.M703643200 |
| 22709581 | X-ray crystal structure and specificity of the Plasmodium falciparum malariaaminopeptidase PfM18AAP. | Sivaraman KK, Oellig CA, ..., McGowan S | 10.1016/j.jmb.2012.06.006 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 30929948 | In silico study of M18 aspartyl amino peptidase (M18AAP) of Plasmodium vivax asan antimalarial drug target | Rout S, Mahapatra RK | 10.1016/j.bmc.2019.03.039 |
| 25869318 | In silico prediction of anti-malarial hit molecules based on machine learningmethods | Kumari M, Chandra S | 10.1504/IJCBDD.2015.068783 |
| 27534744 | 3D QSAR, pharmacophore and molecular docking studies of known inhibitors anddesigning of novel inhibitors for M18 aspartyl aminopeptidase of Plasmodiumfalciparum | Kumari M, Chandra S, Tiwari N, Subbarao N | 10.1186/s12900-016-0063-7 |