Genome location: Pf3D7_09_v3:1,291,568..1,293,643(-)
Genome classification: Core
Product Description: peptide chain release factor 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.1.29 (Aminoacyl-tRNA hydrolase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.98 |
| Trophozoite | expressed | 0.24 | 0.85 |
| Schizont | expressed | 0.17 | 0.89 |
| Gametocyte | expressed | 0.23 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0932400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.938 | MFS: -2.911 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1016100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2J2
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.1.29 | aminoacyl-tRNA hydrolase | EDTAtRNAtRNALystRNAfMettRNA-lysine5-azacytidine70 S ribosomeUncharged tRNAdeacetylated tRNAdiethyldicarbonatenon-esterified tRNALystRNA-formyl-methionine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101033
Most Similar Human Ortholog: Q9UGC7
TM-align score: 0.64 | RMSD: 4.13
Seq Identity: 0.20 | Length: 304 / 404
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000112031 | mitochondrial translational release factor 1 like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 16 | 9 | 20 |
| disruptive | 6 | 21 | 13 | 45 |
| missense | 6 | 20 | 13 | 39 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 31 | 13 | 26 |
| disruptive | 15 | 61 | 31 | 93 |
| missense | 11 | 44 | 27 | 67 |
PlasmoDB Total SNPs: 109
Non-coding: 91 | Synonymous: 11 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 404 | Molecular Weight (kDa): 47.99
UniProt ID(s): Q8I2J2
PDB ID(s): None
Isoelectric Point: 9.46
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000352 | Peptide chain release factor class I |
| InterPro | IPR005139 | Peptide chain release factor |
| PFam | PF00472 | Peptide chain release factor class I |
| PFam | PF03462 | Peptide chain release factor |
| Superfamily | SSF75620 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |