PF3D7_0932500 (DHHC6)

Genome location: Pf3D7_09_v3:1,293,832..1,297,076(-)

Genome classification: Core

Function and Localization

Product Description: palmitoyltransferase DHHC6, putative

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.225 (Protein S-acyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Component	GO:0005783	endoplasmic reticulum
Function	GO:0016409	palmitoyltransferase activity
Function	GO:0019706	protein-cysteine S-palmitoyltransferase activity
Process	GO:0018230	peptidyl-L-cysteine S-palmitoylation
Process	GO:0018345	protein palmitoylation
Process	GO:0006612	protein targeting to membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	expressed	0.44	0.74
Schizont	expressed	0.37	0.77
Gametocyte	expressed	0.52	0.71

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0932500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.335 | MFS: -2.63 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0833300)

Relative Growth Rate: 0.94 ± 0.15
Confidence: 5.14

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0833300)

Modification: Disrupted | RMgm-2456

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2J1

"Best" AlphaFill ligand hit: DYD ((2s,5s)-hexane-2,5-diol, Local RMSD=0.02) with 6BMM (Global RMSD=3.63)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.1.-	Ex: [myelin-proteolipid] O-palmitoyltransferase	CoAKClArgATPADPEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4...
2.3.1.225	protein S-acyltransferase	stearoyl-CoAmyristoyl-CoA2-Bromopalmitate2-bromo-palmitateacyl-CoA binding protein

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104740

Most Similar Human Ortholog: Q96GR4

TM-align score: 0.80 | RMSD: 2.92

Seq Identity: 0.29 | Length: 244 / 553

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000160446	zinc finger DHHC-type palmitoyltransferase 12

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	24	20	27
disruptive	15	45	41	98
missense	12	45	38	87

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	62	25	34
disruptive	26	136	62	144
missense	17	114	50	116

PlasmoDB Total SNPs: 233

Non-coding: 197 | Synonymous: 20 | Nonsynonymous: 16 | Stop Codon: 0

Protein Information

Protein Length: 553 | Molecular Weight (kDa): 67.022

UniProt ID(s): Q8I2J1

PDB ID(s): None

Isoelectric Point: 9.81

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001594	Palmitoyltransferase, DHHC domain
PFam	PF01529	Palmitoyltransferase, DHHC domain
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
23638681	Global analysis of apicomplexan protein S-acyl transferases reveals an enzymeessential for invasion.	Frenal K, Tay CL, ..., Soldati-Favre D	10.1111/tra.12081