Genome location: Pf3D7_09_v3:1,330,167..1,333,406(-)
Genome classification: Core
Product Description: mitochondrial-processing peptidase subunit beta, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.24.64 (Mitochondrial processing peptidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0017087 | mitochondrial processing peptidase complex |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004222 | metalloendopeptidase activity |
| Function | GO:0008233 | peptidase activity |
| Process | GO:0006627 | protein processing involved in protein targeting to mitochondrion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.14 | 0.93 |
| Trophozoite | expressed | 0.58 | 0.66 |
| Schizont | expressed | 0.12 | 0.91 |
| Gametocyte | expressed | 0.34 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0933600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.25 | MFS: -2.745 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0834400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0834400)
Modification: Disrupted | RMgm-2458
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2I2
"Best" AlphaFill ligand hit: PX4 (1,2-dimyristoyl-sn-glycero-3-phosphocholine, Local RMSD=0.01) with 5KLV (Global RMSD=2.07)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.24.64 | mitochondrial processing peptidase | GTPIAAEDTAPCMBRhodaneseMyxothiazolferricyanideantisense RNAdithiothreitolPrepeptide p25Prepeptide p34iodoacetic acid... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100777
Most Similar Human Ortholog: P31930
TM-align score: 0.91 | RMSD: 1.29
Seq Identity: 0.37 | Length: 445 / 484
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000010256 | ubiquinol-cytochrome c reductase core protein 1 |
| ENSG00000105819 | peptidase, mitochondrial processing subunit beta |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 23 | 15 | 36 |
| disruptive | 1 | 8 | 11 | 41 |
| missense | 1 | 8 | 10 | 36 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 54 | 16 | 25 |
| disruptive | 5 | 36 | 23 | 92 |
| missense | 3 | 26 | 20 | 69 |
PlasmoDB Total SNPs: 156
Non-coding: 139 | Synonymous: 14 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 484 | Molecular Weight (kDa): 55.735
PDB ID(s): None
Isoelectric Point: 6.76
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001431 | Peptidase M16, zinc-binding site |
| InterPro | IPR007863 | Peptidase M16, C-terminal |
| InterPro | IPR011249 | Metalloenzyme, LuxS/M16 peptidase-like |
| InterPro | IPR011765 | Peptidase M16, N-terminal |
| PFam | PF00675 | Peptidase M16, N-terminal |
| PFam | PF05193 | Peptidase M16, C-terminal |
| Superfamily | SSF63411 | Metalloenzyme, LuxS/M16 peptidase-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30594502 | Plasmodium falciparum PFI1625c offers an opportunity to design potentanti-malarials: Biochemical characterization and testing potentials in drugdiscovery. | Lhouvum K, Balaji SN, Ahsan MJ, Trivedi V | 10.1016/j.actatropica.2018.12.033 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 30099677 | Genome-wide evidences of bisphenol a toxicity using Schizosaccharomyces pombe | Kim DM, Heo J, ..., Yang M | 10.1007/s12272-018-1058-7 |
| 33576883 | Identification of new proteins related with cisplatin resistance in Saccharomyces cerevisiae | Burgos-Molina AM, Mercado-Saenz S, ..., Ruiz-Gomez MJ | 10.1007/s00253-021-11137-w |