PF3D7_0933800

Genome location: Pf3D7_09_v3:1,335,740..1,337,947(+)

Genome classification: Core

Function and Localization

Product Description: tubulin delta chain, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005874	microtubule
Function	GO:0005525	GTP binding
Function	GO:0005200	structural constituent of cytoskeleton
Process	GO:0000226	microtubule cytoskeleton organization
Process	GO:0007017	microtubule-based process
Process	GO:0000278	mitotic cell cycle

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.99
Trophozoite	not expressed	0.07	0.96
Schizont	possibly expressed	0.06	0.96
Gametocyte	possibly expressed	0.37	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0933800

Resistome Missense Mutations: Q482H

Resistome Compounds with Missense Mutations: Desoxyepothilone B

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.444 | #Insertions: 7

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0834600)

Relative Growth Rate: 1.04 ± 0.09
Confidence: 6.22

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0834600)

Modification: Disrupted | RMgm-2459

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2I0

"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=2.72) with 4LNU (Global RMSD=2.73)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105469

Most Similar Human Ortholog: K7EKE2

TM-align score: 0.91 | RMSD: 1.32

Seq Identity: 0.45 | Length: 96 / 735

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000108423	tubulin delta 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	33	16	39
disruptive	21	44	25	101
missense	14	38	24	90

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	62	29	41
disruptive	30	134	72	223
missense	18	105	55	146

PlasmoDB Total SNPs: 42

Non-coding: 0 | Synonymous: 26 | Nonsynonymous: 16 | Stop Codon: 0

Protein Information

Protein Length: 735 | Molecular Weight (kDa): 86.52

UniProt ID(s): Q8I2I0

PDB ID(s): None

Isoelectric Point: 7.59

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000217	Tubulin
InterPro	IPR002967	Delta tubulin
InterPro	IPR003008	Tubulin/FtsZ, GTPase domain
InterPro	IPR008280	Tubulin/FtsZ, C-terminal
InterPro	IPR036525	Tubulin/FtsZ, GTPase domain superfamily
PFam	PF00091	Tubulin/FtsZ, GTPase domain
Superfamily	SSF52490	Tubulin/FtsZ, GTPase domain superfamily
Superfamily	SSF55307	Tubulin/FtsZ, C-terminal