Product Description: histidine--tRNA ligase, putative
SignalP Peptide: MPLLFFILLIIYFHLIVC
# Transmembrane Domains: 0
EC Numbers: 6.1.1.21 (Histidine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0004812 | aminoacyl-tRNA ligase activity |
| Function | GO:0004821 | histidine-tRNA ligase activity |
| Process | GO:0006427 | histidyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.11 | 0.94 |
| Trophozoite | expressed | 0.55 | 0.68 |
| Schizont | possibly expressed | 0.23 | 0.85 |
| Gametocyte | not expressed | 0.33 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0934000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.872 | MFS: -2.956 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1308900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2H8
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=1.64) with 5E3I (Global RMSD=4.40)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.21 | histidine-tRNA ligase | ADPAMPKClNEMRNAiornithinediphosphateD-histidineL-histidinolDL-homocysteinequinolin-3-amineN-Acetylhistidine... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P12081 | Histidine--tRNA ligase, cytoplasmic | Homo sapiens | OrthoDB | HisSA |
Ortholog Group (OrthoMCL): OG6_100351
Most Similar Human Ortholog: D6RJE6
TM-align score: 0.87 | RMSD: 2.17
Seq Identity: 0.20 | Length: 278 / 642
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000112855 | histidyl-tRNA synthetase 2, mitochondrial |
| ENSG00000170445 | histidyl-tRNA synthetase 1 |
Protein Length: 642 | Molecular Weight (kDa): 78.184
UniProt ID(s): Q8I2H8
PDB ID(s): None
Isoelectric Point: 9.91
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004516 | Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit |
| InterPro | IPR041715 | Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain |
| PFam | PF13393 | Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain |
| Superfamily | SSF52954 | N/A |
| Superfamily | SSF55681 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 26 | 16 | 40 |
| disruptive | 7 | 50 | 29 | 69 |
| missense | 6 | 46 | 27 | 59 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 69 | 18 | 48 |
| disruptive | 19 | 134 | 57 | 120 |
| missense | 13 | 96 | 41 | 80 |
PlasmoDB Total SNPs: 153
Non-coding: 100 | Synonymous: 42 | Nonsynonymous: 11 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |