PF3D7_0934400 (ApiAP2)

Genome location: Pf3D7_09_v3:1,349,597..1,350,572(+)

Genome classification: Core

Function and Localization

Product Description: AP2 domain transcription factor, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.14	0.93
Trophozoite	expressed	0.25	0.83
Schizont	expressed	0.19	0.87
Gametocyte	expressed	0.22	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0934400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 1 | MFS: 0 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0835200)

Relative Growth Rate: 0.12 ± 0.06
Confidence: 6.90

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2H4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_160734

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	4	6	22
disruptive	0	5	8	15
missense	0	5	8	12

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	20	7	24
disruptive	1	17	21	39
missense	1	16	15	29

PlasmoDB Total SNPs: 28

Non-coding: 18 | Synonymous: 10 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 200 | Molecular Weight (kDa): 24.597

UniProt ID(s): Q8I2H4, Q9NFF9

PDB ID(s): None

Isoelectric Point: 10.52

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001471	AP2/ERF domain
PFam	PF00847	AP2/ERF domain
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
34492224	Single-genome sequencing reveals within-host evolution of human malariaparasites	Dia A, Jett C, ..., Cheeseman IH	10.1016/j.chom.2021.08.009
37898053	Cell cycle-regulated ApiAP2s and parasite development: the Toxoplasma paradigm	Zarringhalam K, Ye S, ..., Gubbels MJ	10.1016/j.mib.2023.102383
35087229	The transcriptional regulator HDP1 controls expansion of the inner membranecomplex during early sexual differentiation of malaria parasites	Campelo Morillo RA, Tong X, ..., Kafsack BFC	10.1038/s41564-021-01045-0
35871979	A transcription factor helps Plasmodium falciparum gametocytogenesis take shape	McLean KJ, Niles JC	10.1016/j.pt.2022.07.004
38311493	Sex in a dish: high-efficiency Toxoplasma pre-sexual development	Hryckowian ND, Knoll LJ	10.1016/j.pt.2024.01.009
36292619	Epigenetic and Epitranscriptomic Gene Regulation in Plasmodium falciparum and HowWe Can Use It against Malaria	Serrano-Duran R, Lopez-Farfan D, Gomez-Diaz E	10.3390/genes13101734
37350586	An apicomplexan bromodomain protein, TgBDP1, associates with diverse epigeneticfactors to regulate essential transcriptional processes in Toxoplasma gondii	Fleck K, McNutt S, Chu F, Jeffers V	10.1128/mbio.03573-22
34880324	Essential role of a Plasmodium berghei heat shock protein (PBANKA_0938300) ingametocyte development	Kashif M, Quadiri A, Singh AP	10.1038/s41598-021-03059-4
36847501	Single-Cell Transcriptomics To Define Plasmodium falciparum Stage Transition inthe Mosquito Midgut	Mohammed M, Dziedziech A, ..., Ankarklev J	10.1128/spectrum.03671-22
38370614	Structural characterization of the ACDC domain from ApiAP2 proteins of themalaria parasite	Le Berre M, Tubiana T, ..., Nessler S	10.1101/2024.02.09.579679
35083215	PfAP2-EXP2, an Essential Transcription Factor for the IntraerythrocyticDevelopment of Plasmodium falciparum	Shang X, Wang C, ..., Jiang M	10.3389/fcell.2021.782293
34874774	The Extracellular Milieu of Toxoplasma's Lytic Cycle Drives Lab Adaptation,Primarily by Transcriptional Reprogramming	Primo VA Jr, Rezvani Y, ..., Gubbels MJ	10.1128/mSystems.01196-21
37293082	PfAP2-MRP DNA-binding protein is a master regulator of parasite pathogenesisduring malaria parasite blood stages	Subudhi AK, Green JL, ..., Pain A	10.1101/2023.05.23.541898
36223427	Inhibitors of ApiAP2 protein DNA binding exhibit multistage activity againstPlasmodium parasites	Russell TJ, De Silva EK, ..., Llinas M	10.1371/journal.ppat.1010887
35288749	Genome-wide landscape of ApiAP2 transcription factors reveals aheterochromatin-associated regulatory network during Plasmodium falciparumblood-stage development	Shang X, Wang C, ..., Zhang Q	10.1093/nar/gkac176
34400833	A heat-shock response regulated by the PfAP2-HS transcription factor protectshuman malaria parasites from febrile temperatures	Tinto-Font E, Michel-Todo L, ..., Cortes A	10.1038/s41564-021-00940-w