About Gene List

PF3D7_0934500

Genome location: Pf3D7_09_v3:1,350,428..1,353,738(-)

Genome classification: Core

Function and Localization

Product Description: V-type proton ATPase subunit E, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.3.14 (Transferred entry: 7.1.2.2)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020020 food vacuole
Component GO:0005634 nucleus
Function GO:0008553 P-type proton-exporting transporter activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.18 0.91
Trophozoite expressed 0.27 0.82
Schizont expressed 0.21 0.84
Gametocyte expressed 0.22 0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0934500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.141 | MFS: -2.871 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0835300)

  • Relative Growth Rate: 0.34 ± 0.38
  • Confidence: 3.33

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2H3

"Best" AlphaFill ligand hit: PBM (trimethyl lead ion, Local RMSD=0.07) with 4DL0 (Global RMSD=4.04)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.6.3.14 H+-transporting two-sector ATPase No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101237

Most Similar Human Ortholog: P36543

TM-align score: 0.91 | RMSD: 1.70

Seq Identity: 0.39 | Length: 219 / 235

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000131100 ATPase H+ transporting V1 subunit E1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 0 0 0 0
disruptive 0 1 0 0
missense 0 0 0 0

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 0 0 0 0
disruptive 1 0 0 1
missense 0 0 0 0

PlasmoDB Total SNPs: 172

Non-coding: 167 | Synonymous: 3 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 235 | Molecular Weight (kDa): 27.191

UniProt ID(s): Q8I2H3

PDB ID(s): None

Isoelectric Point: 9.56

Protein Domain Annotations:

Source Family ID Description
InterPro IPR002842 V-type ATPase subunit E
PFam PF01991 V-type ATPase subunit E
Superfamily SSF160527 N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
21136929 Food vacuole proteome of the malarial parasite Plasmodium falciparum. Lamarque M, Tastet C, ..., Dubremetz JF 10.1002/prca.200700112
30379851 Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. Briquet S, Ourimi A, ..., Vaquero C 10.1371/journal.pone.0205596