Genome location: Pf3D7_09_v3:1,366,182..1,367,150(-)
Genome classification: Core
Product Description: U2 small nuclear ribonucleoprotein B'', putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005685 | U1 snRNP |
| Component | GO:0005634 | nucleus |
| Function | GO:0030619 | U1 snRNA binding |
| Function | GO:0003729 | mRNA binding |
| Process | GO:0000398 | mRNA splicing, via spliceosome |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.08 | 0.96 |
| Trophozoite | expressed | 0.21 | 0.87 |
| Schizont | expressed | 0.05 | 0.97 |
| Gametocyte | expressed | 0.04 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0935000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.017 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1405400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2G9
"Best" AlphaFill ligand hit: 15P (peg 1500, Local RMSD=0.08) with 6F4J (Global RMSD=3.54)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100753
Most Similar Human Ortholog: M0R0G9
TM-align score: 0.91 | RMSD: 1.50
Seq Identity: 0.38 | Length: 86 / 193
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000077312 | small nuclear ribonucleoprotein polypeptide A |
| ENSG00000125870 | small nuclear ribonucleoprotein polypeptide B2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 9 | 11 | 17 |
| disruptive | 2 | 8 | 10 | 20 |
| missense | 2 | 8 | 10 | 19 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 21 | 12 | 14 |
| disruptive | 5 | 23 | 20 | 38 |
| missense | 3 | 16 | 18 | 32 |
PlasmoDB Total SNPs: 27
Non-coding: 24 | Synonymous: 1 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 193 | Molecular Weight (kDa): 22.295
UniProt ID(s): Q8I2G9
PDB ID(s): None
Isoelectric Point: 9.63
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000504 | RNA recognition motif domain |
| InterPro | IPR035979 | RNA-binding domain superfamily |
| PFam | PF00076 | RNA recognition motif domain |
| Superfamily | SSF54928 | RNA-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27381095 | The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. | Bunnik EM, Batugedara G, ..., Le Roch KG | 10.1186/s13059-016-1014-0 |
| 9799242 | The yeast U2A'/U2B complex is required for pre-spliceosome formation | Caspary F, Seraphin B | 10.1093/emboj/17.21.6348 |
| 9716128 | Crystal structure of the spliceosomal U2B"-U2A' protein complex bound to afragment of U2 small nuclear RNA | Price SR, Evans PR, Nagai K | 10.1038/29234 |
| 7556097 | Molecular characterization of the spliceosomal proteins U1A and U2B" from higherplants | Simpson GG, Clark GP, ..., Brown JW | 10.1002/j.1460-2075.1995.tb00133.x |