About Gene List

PF3D7_0935200 (VPS33)

Genome location: Pf3D7_09_v3:1,368,257..1,372,877(-)

Genome classification: Core

Function and Localization

Product Description: vacuolar protein sorting-associated protein 33, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005886 plasma membrane
Component GO:0030141 secretory granule
Function GO:0019905 syntaxin binding
Process GO:0006886 intracellular protein transport
Process GO:0006904 vesicle docking involved in exocytosis
Process GO:0016192 vesicle-mediated transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.67 0.69
Trophozoite expressed 1.11 0.45
Schizont expressed 0.57 0.70
Gametocyte possibly expressed 1.13 0.44

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0935200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.124 | MFS: -3.101 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0836000)

  • Relative Growth Rate: 0.15 ± 0.19
  • Confidence: 4.70

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2G8

"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=6.60) with 3LQQ (Global RMSD=12.81)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101460

Most Similar Human Ortholog: Q96AX1

TM-align score: 0.89 | RMSD: 2.92

Seq Identity: 0.27 | Length: 575 / 1147

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000139719 VPS33A core subunit of CORVET and HOPS complexes
ENSG00000256861 novel protein

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 9 48 28 55
disruptive 92 234 91 203
missense 44 120 58 116

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 24 84 37 63
disruptive 159 370 135 267
missense 74 206 88 158

PlasmoDB Total SNPs: 219

Non-coding: 78 | Synonymous: 77 | Nonsynonymous: 64 | Stop Codon: 0

Protein Information

Protein Length: 1147 | Molecular Weight (kDa): 135.978

UniProt ID(s): Q8I2G8

PDB ID(s): None

Isoelectric Point: 4.56

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001619 Sec1-like protein
InterPro IPR027121 Vacuolar protein sorting-associated protein 33
InterPro IPR036045 Sec1-like superfamily
PFam PF00995 Sec1-like protein
Superfamily SSF56815 Sec1-like superfamily

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095