Genome location: Pf3D7_09_v3:1,412,562..1,419,940(+)
Genome classification: Core
Product Description: cytoadherence linked asexual protein 9
SignalP Peptide: MIIWFIQPTIFYIIFILA
# Transmembrane Domains: 2
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0045177 | apical part of cell |
| Component | GO:0005634 | nucleus |
| Component | GO:0020008 | rhoptry |
| Function | GO:0003723 | RNA binding |
| Function | GO:0046789 | host cell surface receptor binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0098609 | cell-cell adhesion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.97 |
| Trophozoite | not expressed | 0.24 | 0.87 |
| Schizont | expressed | 1.61 | 0.35 |
| Gametocyte | not expressed | 0.04 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0935800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.138 | MFS: -2.922 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1400600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2G2
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 26 | 19 | 40 |
| disruptive | 35 | 41 | 27 | 69 |
| missense | 35 | 39 | 27 | 54 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 54 | 31 | 62 |
| disruptive | 43 | 92 | 64 | 167 |
| missense | 42 | 75 | 54 | 123 |
PlasmoDB Total SNPs: 275
Non-coding: 213 | Synonymous: 35 | Nonsynonymous: 27 | Stop Codon: 0
Protein Length: 1340 | Molecular Weight (kDa): 160.417
UniProt ID(s): B0M0V8, B0M157, B0M164, O77090, Q4AE83, Q8I2G2, Q9GQ95, Q9GQ97, Q9GQ98, Q9GQ99, Q9GQA0, Q9GQA1, Q9NAV3, Q9NAV4
PDB ID(s): None
Isoelectric Point: 9.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005553 | Cytoadherence-linked asexual protein |
| PFam | PF03805 | Cytoadherence-linked asexual protein |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10737759 | clag9: A cytoadherence gene in Plasmodium falciparum essential for binding ofparasitized erythrocytes to CD36. | Trenholme KR, Gardiner DL, ..., Kemp DJ | 10.1073/pnas.040561197 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 20823248 | Dual stage synthesis and crucial role of cytoadherence-linked asexual gene 9 inthe surface expression of malaria parasite var proteins. | Goel S, Valiyaveettil M, ..., Gowda DC | 10.1073/pnas.1002568107 |
| 28252383 | Plasmodium falciparum parasites deploy Rhop H2 into the host erythrocyte to obtainnutrients, grow and replicate | Counihan NA, Chisholm SA, ..., de Koning-Ward TF | 10.7554/eLife.23217 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31712270 | Plasmodium falciparum Clag9-Associated Pf RhopH Complex Is Involved in Merozoite Binding to Human Erythrocytes | Chourasia BK, Deshmukh A, ..., Malhotra P | 10.1128/IAI.00504-19 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 11163449 | The sequence of clag 9, a subtelomeric gene of plasmodium falciparum is highlyconserved | Manski-Nankervis JA, Gardiner DL, ..., Trenholme KR | 10.1016/s0166-6851(00)00323-6 |
| 23264726 | Stable Allele Frequency Distribution of the Plasmodium falciparum clag GenesEncoding Components of the High Molecular Weight Rhoptry Protein Complex | Alexandre JS, Xangsayarath P, ..., Kaneko O | 10.2149/tmh.2012-13 |
| 15964954 | Antibody reactivity to linear epitopes of Plasmodium falciparumcytoadherence-linked asexual gene 9 in asymptomatic children and adults frompapua new Guinea | Trenholme KR, Boutlis CS, ..., Gardiner DL | https://pubmed.ncbi.nlm.nih.gov/15964954/ |
| 15049814 | The Plasmodium falciparum clag9 gene encodes a rhoptry protein that istransferred to the host erythrocyte upon invasion | Ling IT, Florens L, ..., Mattei D | 10.1111/j.1365-2958.2003.03969.x |
| 10989143 | Inhibition of Plasmodium falciparum clag9 gene function by antisense RNA | Gardiner DL, Holt DC, ..., Trenholme KR | 10.1016/s0166-6851(00)00254-1 |
| 32069335 | Complex nutrient channel phenotypes despite Mendelian inheritance in a Plasmodiumfalciparum genetic cross | Gupta A, Bokhari AAB, ..., Desai SA | 10.1371/journal.ppat.1008363 |
| 22028613 | Positive selection on the Plasmodium falciparum clag2 gene encoding a componentof the erythrocyte-binding rhoptry protein complex | Alexandre JS, Kaewthamasorn M, ..., Kaneko O | 10.2149/tmh.2011-12 |
| 15953647 | Apical expression of three RhopH1/Clag proteins as components of the Plasmodiumfalciparum RhopH complex | Kaneko O, Yim Lim BY, ..., Torii M | https://pubmed.ncbi.nlm.nih.gov/15953647/ |
| 22205992 | Clag9 is not essential for PfEMP1 surface expression in non-cytoadherentPlasmodium falciparum parasites with a chromosome 9 deletion | Nacer A, Roux E, ..., Mattei D | 10.1371/journal.pone.0029039 |
| 15103554 | CLAG 9 is located in the rhoptries of Plasmodium falciparum | Gardiner DL, Spielmann T, ..., Trenholme KR | 10.1007/s00436-004-1098-4 |
| 11738716 | Clags in Plasmodium falciparum and other species of Plasmodium | Holt DC, Fischer K, ..., Kemp DJ | 10.1016/s0166-6851(01)00378-4 |
| 20085836 | Sequences of the Plasmodium falciparum cytoadherence-linked asexual protein 9implicated in malaria parasite invasion to erythrocytes | Pinzon CG, Curtidor H, ..., Patarroyo ME | 10.1016/j.vaccine.2010.01.004 |
| 10951593 | Malaria: a new gene family (clag) involved in adhesion | Craig A | 10.1016/s0169-4758(00)01744-0 |
| 23440122 | Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasitecarriers of Plasmodium vivax | Costa JD, Zanchi FB, ..., Pereira-da-Silva LH | 10.1590/s0074-02762013000100016 |