PF3D7_0936700

Genome location: Pf3D7_09_v3:1,454,422..1,455,720(+)

Genome classification: Core

Function and Localization

Product Description: lysophospholipase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.1.5 (Lysophospholipase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016020	membrane
Function	GO:0016298	lipase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	not expressed	0.01	0.99
Schizont	not expressed	0.01	1.00
Gametocyte	not expressed	0.00	1.00

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0936700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.132 | MFS: -3.109 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1220300)

Relative Growth Rate: 1.09 ± 0.18
Confidence: 4.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2F3

"Best" AlphaFill ligand hit: SRT (s,r meso-tartaric acid, Local RMSD=3.06) with 6T5O (Global RMSD=15.96)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.1.5	lysophospholipase	NEMSDSEDTAPCMBEGTAPMSFDMSOCaCl2FeCl3xyleneethanolsulfite...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100231

Most Similar Human Ortholog: A0A3B3ITT3

TM-align score: 0.88 | RMSD: 2.39

Seq Identity: 0.23 | Length: 252 / 432

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000074416	monoglyceride lipase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	22	10	19
disruptive	35	70	55	84
missense	25	47	39	64

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	32	16	27
disruptive	53	159	76	129
missense	38	112	53	81

PlasmoDB Total SNPs: 88

Non-coding: 0 | Synonymous: 47 | Nonsynonymous: 41 | Stop Codon: 0

Protein Information

Protein Length: 432 | Molecular Weight (kDa): 49.555

UniProt ID(s): Q8I2F3

PDB ID(s): None

Isoelectric Point: 4.51

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006494	Plasmodium subtelomeric PST-A
InterPro	IPR022742	Serine aminopeptidase, S33
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	PF12146	Serine aminopeptidase, S33
Superfamily	SSF53474	Alpha/Beta hydrolase fold