About Gene List

PF3D7_1000500 (RIF)

Genome location: Pf3D7_10_v3:48,052..49,384(-)

Genome classification: SubtelomericHypervariable

Function and Localization

Product Description: rifin

SignalP Peptide: MKVHYFNILLFALPLNILVSSP

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020036 Maurer's cleft
Component GO:0020002 host cell plasma membrane
Component GO:0020003 symbiont-containing vacuole
Process GO:0020033 antigenic variation
Process GO:0020035 cytoadherence to microvasculature, mediated by symbiont protein
Process GO:0020013 modulation by symbiont of host erythrocyte aggregation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.00 1.00
Trophozoite expressed 0.00 1.00
Schizont possibly expressed 0.00 1.00
Gametocyte possibly expressed 0.00 1.00

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1000500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.506 | #Insertions: 6

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A143ZWD5

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100719

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 568

Non-coding: 61 | Synonymous: 128 | Nonsynonymous: 376 | Stop Codon: 3

Protein Information

Protein Length: 369 | Molecular Weight (kDa): 40.963

UniProt ID(s): A0A143ZWD5

PDB ID(s): 7F9M, 7F9N

Isoelectric Point: 9.74

Protein Domain Annotations:

Source Family ID Description
InterPro IPR006373 Variant surface antigen Rifin
InterPro IPR011992 EF-hand domain pair
PFam PF02009 Variant surface antigen Rifin
Superfamily SSF47473 EF-hand domain pair

Associated Publications

PMID Title Authors DOI/Link
10430943 Rifins: a second family of clonally variant proteins expressed on the surface ofred cells infected with Plasmodium falciparum. Kyes SA, Rowe JA, Kriek N, Newbold CI 10.1073/pnas.96.16.9333
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
25751816 RIFINs are adhesins implicated in severe Plasmodium falciparum malaria. Goel S, Palmkvist M, ..., Wahlgren M 10.1038/nm.3812