About Gene List

PF3D7_1001600 (XL2)

Genome location: Pf3D7_10_v3:85,910..91,177(-)

Genome classification: Core

Function and Localization

Product Description: exported lipase 2

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.1.23 (Acylglycerol lipase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0044164 host cell cytosol
Component GO:0016020 membrane
Function GO:0016298 lipase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring possibly expressed 0.22 0.90
Trophozoite expressed 1.33 0.45
Schizont expressed 1.67 0.39
Gametocyte not expressed 0.06 0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1001600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.009 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IK20

"Best" AlphaFill ligand hit: D10 (decane, Local RMSD=1.98) with 8B9S (Global RMSD=7.24)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.1.1.23 acylglycerol lipase BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_157547

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 5 17 11 45
disruptive 19 52 29 69
missense 17 49 28 64

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 8 37 24 48
disruptive 32 109 55 123
missense 26 95 46 95

PlasmoDB Total SNPs: 208

Non-coding: 144 | Synonymous: 44 | Nonsynonymous: 18 | Stop Codon: 2

Protein Information

Protein Length: 763 | Molecular Weight (kDa): 88.564

UniProt ID(s): Q8IK20

PDB ID(s): None

Isoelectric Point: 7.06

Protein Domain Annotations:

Source Family ID Description
InterPro IPR022742 Serine aminopeptidase, S33
InterPro IPR029058 Alpha/Beta hydrolase fold
PFam PF12146 Serine aminopeptidase, S33
Superfamily SSF53474 Alpha/Beta hydrolase fold

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
27795395 Exported Epoxide Hydrolases Modulate Erythrocyte Vasoactive Lipids during Plasmodium falciparum Infection Spillman NJ, Dalmia VK, Goldberg DE 10.1128/mBio.01538-16
37131712 Metabolism of host lysophosphatidylcholine in Plasmodium falciparum-infectederythrocytes Liu J, Dapper C, Klemba M 10.1101/2023.04.17.537066
38349879 Metabolism of host lysophosphatidylcholine in Plasmodium falciparum-infectederythrocytes Liu J, Fike KR, Dapper C, Klemba M 10.1073/pnas.2320262121
36314721 Peptide-based chemical models for lytic polysaccharide monooxygenases Hassoon AA, Szorcsik A, ..., Gajda T 10.1039/d2dt02836k