PF3D7_1004300 (RFUL)

Genome location: Pf3D7_10_v3:198,954..204,653(-)

Genome classification: Core

Function and Localization

Product Description: RING finger E3 ubiquitin-protein ligase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.3.2.27 (RING-type E3 ubiquitin transferase);6.3.2.- (Acid--amino-acid ligases (peptide synthases).)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0061630	ubiquitin protein ligase activity
Process	GO:0016567	protein ubiquitination
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.90	0.59
Trophozoite	expressed	0.74	0.60
Schizont	expressed	0.70	0.60
Gametocyte	expressed	0.83	0.56

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1004300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.986 | MFS: -2.039 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1202600)

Relative Growth Rate: 0.79 ± 0.26
Confidence: 4.06

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1202600)

Modification: Disrupted | RMgm-3176

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJZ4

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.2.27	RING-type E3 ubiquitin transferase	EDTADTPAMLN4924RAPTA-CRAPTA-TRAPTA-EA1
6.3.2.-	Ex: enterobactin synthase	ADPAMPPCMBpABANH4+D-SerATP4-MurEP1BorateacetateformateButyrate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105543

Most Similar Human Ortholog: Q9P0P0

TM-align score: 0.69 | RMSD: 2.06

Seq Identity: 0.34 | Length: 117 / 1130

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000168894	ring finger protein 181

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	19	84	38	82
disruptive	61	220	123	225
missense	32	147	95	170

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	34	151	56	92
disruptive	128	420	183	359
missense	60	308	139	229

PlasmoDB Total SNPs: 305

Non-coding: 146 | Synonymous: 91 | Nonsynonymous: 67 | Stop Codon: 1

Protein Information

Protein Length: 1130 | Molecular Weight (kDa): 131.698

UniProt ID(s): Q8IJZ4

PDB ID(s): None

Isoelectric Point: 4.9

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001841	Zinc finger, RING-type
PFam	PF13639	Zinc finger, RING-type
Superfamily	SSF57850	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108