PF3D7_1005000 (MRSapi)

Genome location: Pf3D7_10_v3:225,274..228,144(-)

Genome classification: Core

Function and Localization

Product Description: methionine--tRNA ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 6.1.1.10 (Methionine--tRNA ligase)

Curated GO (PlasmoDB):

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

More info:

• Malaria Parasite Metabolic Pathways (MPMP) Link
• BiGG Metabolic Model Link
• STRING Interaction Network Link

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1005000

Resistome Missense Mutations: L526F

Resistome Compounds with Missense Mutations: BI-2536

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.165 | MFS: -2.777 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I716

"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=0.21) with 3KFL (Global RMSD=2.64)

BRENDA EC Inhibitors:

Orthology to BindingDB Entries:

Orthology Information

Ortholog Group (OrthoMCL): OG6_101119

Most Similar Human Ortholog: Q96GW9

TM-align score: 0.78 | RMSD: 2.57

Seq Identity: 0.27 | Length: 498 / 749

All Human Orthologs (OrthoMCL):

Protein Information

Protein Length: 749 | Molecular Weight (kDa): 89.307

UniProt ID(s): Q8I716

PDB ID(s): None

Isoelectric Point: 9.31

Protein Domain Annotations:

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

Any inclusion in genotype call

PlasmoDB Total SNPs: 112

Non-coding: 25 | Synonymous: 45 | Nonsynonymous: 42 | Stop Codon: 0