PF3D7_1005600

Genome location: Pf3D7_10_v3:243,559..247,064(-)

Genome classification: Core

Function and Localization

Product Description: DnaJ protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.47	0.78
Trophozoite	expressed	0.64	0.65
Schizont	expressed	0.35	0.79
Gametocyte	expressed	0.70	0.62

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1005600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.765 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1203800)

Relative Growth Rate: 1.07 ± 0.11
Confidence: 5.85

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1203800)

Modification: Disrupted | RMgm-3181

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6S3C2

"Best" AlphaFill ligand hit: CLQ (n4-(7-chloro-quinolin-4-yl)-n1,n1-diethyl-pentane-1,4-diamine, Local RMSD=0.89) with 7DI7 (Global RMSD=22.67)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102227

Most Similar Human Ortholog: Q5F1R6

TM-align score: 0.47 | RMSD: 5.61

Seq Identity: 0.27 | Length: 325 / 796

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000168724	DnaJ heat shock protein family (Hsp40) member C21

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	44	20	44
disruptive	81	200	102	197
missense	32	126	80	131

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	21	73	30	55
disruptive	147	368	144	274
missense	59	238	103	166

PlasmoDB Total SNPs: 301

Non-coding: 129 | Synonymous: 94 | Nonsynonymous: 78 | Stop Codon: 0

Protein Information

Protein Length: 796 | Molecular Weight (kDa): 94.684

UniProt ID(s): C6S3C2

PDB ID(s): None

Isoelectric Point: 7.06

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001623	DnaJ domain
InterPro	IPR013087	Zinc finger C2H2-type
InterPro	IPR018253	DnaJ domain, conserved site
InterPro	IPR036236	Zinc finger C2H2 superfamily
InterPro	IPR036869	Chaperone J-domain superfamily
PFam	PF00226	DnaJ domain
PFam	PF12874
Superfamily	SSF46565	Chaperone J-domain superfamily
Superfamily	SSF57667	Zinc finger C2H2 superfamily