About Gene List

PF3D7_1006100 (NOT5)

Genome location: Pf3D7_10_v3:258,913..261,461(-)

Genome classification: Core

Function and Localization

Product Description: CCR4-NOT transcription complex subunit 5, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0030014 CCR4-NOT complex

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.02 0.99
Trophozoite expressed 0.02 0.99
Schizont possibly expressed 0.01 0.99
Gametocyte expressed 0.23 0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1006100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.882 | MFS: -2.351 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A143ZX72

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101346

Most Similar Human Ortholog: A0A0G2JPP0

TM-align score: 0.91 | RMSD: 1.48

Seq Identity: 0.47 | Length: 91 / 178

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000088038 CCR4-NOT transcription complex subunit 3
ENSG00000273943 CCR4-NOT transcription complex subunit 3
ENSG00000274176 CCR4-NOT transcription complex subunit 3
ENSG00000274616 CCR4-NOT transcription complex subunit 3
ENSG00000274941 CCR4-NOT transcription complex subunit 3
ENSG00000275979 CCR4-NOT transcription complex subunit 3
ENSG00000276082 CCR4-NOT transcription complex subunit 3
ENSG00000277114 CCR4-NOT transcription complex subunit 3
ENSG00000277600 CCR4-NOT transcription complex subunit 3
ENSG00000277615 CCR4-NOT transcription complex subunit 3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 0 0 0 1
disruptive 0 1 0 0
missense 0 0 0 0

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 0 0 0 1
disruptive 0 1 0 0
missense 0 0 0 0

PlasmoDB Total SNPs: 182

Non-coding: 176 | Synonymous: 6 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 178 | Molecular Weight (kDa): 21.553

UniProt ID(s): A0A143ZX72

PDB ID(s): None

Isoelectric Point: 9.54

Protein Domain Annotations:

Source Family ID Description
InterPro IPR007282 NOT2/NOT3/NOT5, C-terminal
InterPro IPR040168 Not2/Not3/Not5
PFam PF04153 NOT2/NOT3/NOT5, C-terminal
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
30703164 Plasmodium male gametocyte development and transmission are critically regulatedby the two putative deadenylases of the CAF1/CCR4/NOT complex. Hart KJ, Oberstaller J, ..., Lindner SE 10.1371/journal.ppat.1007164
24427169 The CAF1-NOT complex of trypanosomes Erben E, Chakraborty C, Clayton C 10.3389/fgene.2013.00299
19107399 Carbon source dependent dynamics of the Ccr4-Not complex in Saccharomycescerevisiae Norbeck J 10.1007/s12275-008-0122-2
18442996 A role for Caf1 in mRNA deadenylation and decay in trypanosomes and human cells Schwede A, Ellis L, ..., Clayton C 10.1093/nar/gkn108
24121231 Structure and RNA-binding properties of the Not1-Not2-Not5 module of the yeastCcr4-Not complex Bhaskar V, Roudko V, ..., Conti E 10.1038/nsmb.2686
22169356 Screening for yeast mutants defective in recipient ability for transkingdomconjugation with Escherichia coli revealed importance of vacuolar ATPase activityin the horizontal DNA transfer phenomenon Mizuta M, Satoh E, ..., Suzuki K 10.1016/j.micres.2011.10.001