PF3D7_1007700 (AP2-I)

Genome location: Pf3D7_10_v3:300,187..306,443(-)

Genome classification: Core

Function and Localization

Product Description: AP2 domain transcription factor AP2-I

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020036	Maurer's cleft
Component	GO:0005634	nucleus
Component	GO:0032991	protein-containing complex
Function	GO:0005515	protein binding
Function	GO:0043565	sequence-specific DNA binding
Process	GO:0009410	response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.14	0.93
Trophozoite	expressed	0.83	0.55
Schizont	expressed	0.45	0.73
Gametocyte	expressed	0.58	0.68

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1007700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.474 | MFS: -2.524 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJW6

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532456

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	19	92	53	144
disruptive	53	234	119	310
missense	23	153	90	236

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	36	212	95	111
disruptive	117	499	251	557
missense	54	364	183	356

PlasmoDB Total SNPs: 227

Non-coding: 40 | Synonymous: 139 | Nonsynonymous: 48 | Stop Codon: 0

Protein Information

Protein Length: 1597 | Molecular Weight (kDa): 182.665

UniProt ID(s): Q25770, Q25773, Q8IJW6

PDB ID(s): None

Isoelectric Point: 6.18

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001471	AP2/ERF domain
PFam	PF00847	AP2/ERF domain
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
21060817	Identification and genome-wide prediction of DNA binding specificities for theApiAP2 family of regulators from the malaria parasite.	Campbell TL, De Silva EK, ..., Llinas M	10.1371/journal.ppat.1001165
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
28618269	Red Blood Cell Invasion by the Malaria Parasite Is Coordinated by the PfAP2-ITranscription Factor.	Santos JM, Josling G, ..., Llinas M	10.1016/j.chom.2017.05.006
29326268	Mapping the malaria parasite druggable genome by using in vitro evolution andchemogenomics.	Cowell AN, Istvan ES, ..., Winzeler EA	10.1126/science.aan4472
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596
31631203	A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum.	Das D, Krishnan SR, Roy A, Bulusu G	10.1039/c9mo00124g
32198457	Dissecting the role of PfAP2-G in malaria gametocytogenesis.	Josling GA, Russell TJ, ..., Llinas M	10.1038/s41467-020-15026-0
34261498	MALBoost: a web-based application for gene regulatory network analysis inPlasmodium falciparum	van Wyk R, van Biljon R, Birkholtz LM	10.1186/s12936-021-03848-2
36704725	In silico Structure Prediction, Molecular Docking, and Dynamic Simulation ofPlasmodium falciparum AP2-I Transcription Factor	Oladejo DO, Duselu GO, ..., Adebiyi E	10.1177/11779322221149616
37349497	A type II protein arginine methyltransferase regulates merozoite invasion inPlasmodium falciparum	Lucky AB, Wang C, ..., Miao J	10.1038/s42003-023-05038-z
33272860	The human malaria parasite genome is configured into thousands of coexpressedlinear regulatory units	Wang C, Gibbons J, ..., Jiang RHY	10.1016/j.jgg.2020.08.005
34403450	A unique GCN5 histone acetyltransferase complex controls erythrocyte invasion andvirulence in the malaria parasite Plasmodium falciparum	Miao J, Wang C, ..., Cui L	10.1371/journal.ppat.1009351