Product Description: flap endonuclease, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.2.99.18 (DNA-(apurinic or apyrimidinic site) lyase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0008409 | 5'-3' exonuclease activity |
| Function | GO:0017108 | 5'-flap endonuclease activity |
| Function | GO:0048256 | flap endonuclease activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.99 |
| Trophozoite | not expressed | 0.30 | 0.81 |
| Schizont | not expressed | 0.44 | 0.73 |
| Gametocyte | not expressed | 0.33 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1008200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.995 | MFS: -2.71 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1206400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1206400)
Modification: Disrupted | RMgm-3194
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJW1
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.2.99.18 | DNA-(apurinic or apyrimidinic site) lyase | ATPAMPKClCMPUMPP53EDTAdCMPdTMPdAMPdGMPATCA... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 17 | 18 | 24 |
| disruptive | 2 | 15 | 11 | 38 |
| missense | 2 | 15 | 10 | 35 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 45 | 22 | 26 |
| disruptive | 9 | 48 | 38 | 61 |
| missense | 3 | 40 | 34 | 39 |
PlasmoDB Total SNPs: 42
Non-coding: 13 | Synonymous: 25 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 388 | Molecular Weight (kDa): 45.596
UniProt ID(s): Q8IJW1
PDB ID(s): None
Isoelectric Point: 9.75
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006084 | XPG/Rad2 endonuclease |
| InterPro | IPR006086 | XPG-I domain |
| InterPro | IPR008918 | Helix-hairpin-helix motif, class 2 |
| InterPro | IPR029060 | PIN-like domain superfamily |
| InterPro | IPR036279 | 5'-3' exonuclease, C-terminal domain superfamily |
| PFam | PF00867 | XPG-I domain |
| Superfamily | SSF47807 | 5'-3' exonuclease, C-terminal domain superfamily |
| Superfamily | SSF88723 | PIN-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 8944766 | Computational method to predict mitochondrially imported proteins and theirtargeting sequences. | Claros MG, Vincens P | 10.1111/j.1432-1033.1996.00779.x |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |