Product Description: 26S protease regulatory subunit 4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.8 (Transferred entry: 5.6.1.5)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0005838 | proteasome regulatory particle |
| Component | GO:0008540 | proteasome regulatory particle, base subcomplex |
| Function | GO:0017025 | TBP-class protein binding |
| Function | GO:0004175 | endopeptidase activity |
| Function | GO:0036402 | proteasome-activating activity |
| Process | GO:0070682 | proteasome regulatory particle assembly |
| Process | GO:0006508 | proteolysis |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.22 | 0.89 |
| Trophozoite | expressed | 0.73 | 0.59 |
| Schizont | expressed | 0.64 | 0.62 |
| Gametocyte | expressed | 0.42 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1008400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.745 | MFS: -2.429 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1206600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJW0
"Best" AlphaFill ligand hit: 08T ([[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium, Local RMSD=0.04) with 3U5Z (Global RMSD=6.98)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.8 | proteasome ATPase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101477
Most Similar Human Ortholog: G3V4X1
TM-align score: 0.89 | RMSD: 1.31
Seq Identity: 0.82 | Length: 84 / 448
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000100764 | proteasome 26S subunit, ATPase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 23 | 13 | 44 |
| disruptive | 1 | 4 | 3 | 7 |
| missense | 1 | 4 | 3 | 4 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 64 | 19 | 25 |
| disruptive | 2 | 13 | 22 | 65 |
| missense | 1 | 10 | 17 | 46 |
PlasmoDB Total SNPs: 142
Non-coding: 120 | Synonymous: 18 | Nonsynonymous: 2 | Stop Codon: 2
Protein Length: 448 | Molecular Weight (kDa): 49.839
UniProt ID(s): Q8IJW0
PDB ID(s): None
Isoelectric Point: 7.4
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR003960 | ATPase, AAA-type, conserved site |
| InterPro | IPR005937 | 26S proteasome regulatory subunit P45-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR032501 | Proteasomal ATPase OB C-terminal domain |
| InterPro | IPR041569 | AAA ATPase, AAA+ lid domain |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF16450 | Proteasomal ATPase OB C-terminal domain |
| PFam | PF17862 | AAA ATPase, AAA+ lid domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 34571878 | alpha-Synuclein Decreases the Abundance of Proteasome Subunits and Alters UbiquitinConjugates in Yeast | Popova B, Galka D, ..., Braus GH | 10.3390/cells10092229 |
| 38019907 | Lipid-anchored proteasomes control membrane protein homeostasis | Zhang R, Pan S, ..., Guo X | 10.1126/sciadv.adj4605 |
| 37214852 | Lipid-anchored Proteasomes Control Membrane Protein Homeostasis | Zhang R, Pan S, ..., Guo X | 10.1101/2023.05.12.540509 |