About Gene List

PF3D7_1008700

Genome location: Pf3D7_10_v3:359,585..364,762(-)

Genome classification: Core

Function and Localization

Product Description: tubulin beta chain

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0045177 apical part of cell
Component GO:0009986 cell surface
Component GO:0005737 cytoplasm
Component GO:0020020 food vacuole
Component GO:0005874 microtubule
Component GO:0005634 nucleus
Function GO:0005525 GTP binding
Function GO:0005515 protein binding
Function GO:0005200 structural constituent of cytoskeleton
Process GO:0000226 microtubule cytoskeleton organization
Process GO:0007017 microtubule-based process
Process GO:0000278 mitotic cell cycle
Process GO:0042493 response to drug
Process GO:0009410 response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.14 0.93
Trophozoite expressed 0.90 0.53
Schizont expressed 0.72 0.60
Gametocyte expressed 1.90 0.23

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1008700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.305 | MFS: -2.901 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1206900)

  • Relative Growth Rate: 0.07 ± 0.08
  • Confidence: 6.39

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q7KQL5

"Best" AlphaFill ligand hit: GTP (guanosine-5'-triphosphate, Local RMSD=0.05) with 7YHN (Global RMSD=1.16)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P68371 Tubulin beta-4B chain HOGENOM, OMA, OrthoDB CHEMBL383775
P07437 Tubulin beta chain HOGENOM, OrthoDB 7alphaH-colchicineCHEMBL4128424CHEMBL4127437CHEMBL4129198
Q9H4B7 Tubulin beta-1 chain Homo sapiens HOGENOM, OrthoDB CHEMBL189189combretastatin A-4, (CSA4)CHEMBL364123CHEMBL363063...
Q6B856 Tubulin beta-2B chain Bos taurus OrthoDB CHEMBL1627439CHEMBL14412CHEMBL14644CHEMBL273990...
Q13509 Tubulin beta-3 chain OrthoDB combretastatin A-4, (CSA4)CHEMBL2425924PACLITAXELCHEMBL1824506...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100137

Most Similar Human Ortholog: Q9BUF5

TM-align score: 1.00 | RMSD: 0.57

Seq Identity: 0.84 | Length: 445 / 445

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000101162 tubulin beta 1 class VI
ENSG00000104833 tubulin beta 4A class IVa
ENSG00000137267 tubulin beta 2A class IIa
ENSG00000137285 tubulin beta 2B class IIb
ENSG00000173213 tubulin beta 8B
ENSG00000176014 tubulin beta 6 class V
ENSG00000183311 tubulin beta class I
ENSG00000188229 tubulin beta 4B class IVb
ENSG00000196230 tubulin beta class I
ENSG00000198211 novel protein (MC1R-TUBB3 readthrough)
ENSG00000224156 tubulin beta class I
ENSG00000227739 tubulin beta class I
ENSG00000229684 tubulin beta class I
ENSG00000232421 tubulin beta class I
ENSG00000232575 tubulin beta class I
ENSG00000235067 tubulin beta class I
ENSG00000258947 tubulin beta 3 class III
ENSG00000261456 tubulin beta 8 class VIII

Protein Information

Protein Length: 445 | Molecular Weight (kDa): 49.751

UniProt ID(s): A0A143ZWL7, P14140, Q25792, Q7KQL5

PDB ID(s): None

Isoelectric Point: 4.46

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000217 Tubulin
InterPro IPR002453 Beta tubulin
InterPro IPR003008 Tubulin/FtsZ, GTPase domain
InterPro IPR008280 Tubulin/FtsZ, C-terminal
InterPro IPR013838 Beta tubulin, autoregulation binding site
InterPro IPR017975 Tubulin, conserved site
InterPro IPR018316 Tubulin/FtsZ, 2-layer sandwich domain
InterPro IPR036525 Tubulin/FtsZ, GTPase domain superfamily
PFam PF00091 Tubulin/FtsZ, GTPase domain
PFam PF03953 Tubulin/FtsZ, 2-layer sandwich domain
Superfamily SSF52490 Tubulin/FtsZ, GTPase domain superfamily
Superfamily SSF55307 Tubulin/FtsZ, C-terminal

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 0 0 0 0
disruptive 0 1 0 0
missense 0 0 0 0

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 0 0 0 0
disruptive 1 0 0 1
missense 0 0 0 0

PlasmoDB Total SNPs: 238

Non-coding: 227 | Synonymous: 10 | Nonsynonymous: 1 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
16406111 Cellular and molecular actions of dinitroaniline and phosphorothioamidateherbicides on Plasmodium falciparum: tubulin as a specific antimalarial target. Fennell BJ, Naughton JA, Dempsey E, Bell A 10.1016/j.molbiopara.2005.08.020
21136929 Food vacuole proteome of the malarial parasite Plasmodium falciparum. Lamarque M, Tastet C, ..., Dubremetz JF 10.1002/prca.200700112
22446744 Mapping the genome of Plasmodium falciparum on the drug-like chemical spacereveals novel anti-malarial targets and potential drug leads. Jensen K, Plichta D, Panagiotou G, Kouskoumvekaki I 10.1039/c2mb00008c
30379851 Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. Briquet S, Ourimi A, ..., Vaquero C 10.1371/journal.pone.0205596
33633135 Interaction of Plasmodium falciparum apicortin with alpha- and beta-tubulin is criticalfor parasite growth and survival. Chakrabarti M, Joshi N, ..., Singh S 10.1038/s41598-021-83513-5