Product Description: diphthine methyl ester synthase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.314 (Diphthine methyl ester synthase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Function | GO:0004164 | diphthine synthase activity |
Process | GO:0017183 | peptidyl-diphthamide biosynthetic process from peptidyl-histidine |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | possibly expressed | N/A | N/A |
Ring | expressed | 0.32 | 0.84 |
Trophozoite | expressed | 0.25 | 0.84 |
Schizont | expressed | 0.04 | 0.98 |
Gametocyte | expressed | 0.11 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1009000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.907 | MFS: -2.095 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJV5
"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.60) with 2DV4 (Global RMSD=2.19)
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
2.1.1.314 | diphthine methyl ester synthase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101531
Most Similar Human Ortholog: Q9H2P9
TM-align score: 0.96 | RMSD: 1.41
Seq Identity: 0.46 | Length: 272 / 274
All Human Orthologs (OrthoMCL):
Gene ID | Description |
---|---|
ENSG00000117543 | diphthamide biosynthesis 5 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 4 | 12 | 12 | 23 |
disruptive | 2 | 19 | 15 | 38 |
missense | 2 | 19 | 15 | 37 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 4 | 30 | 17 | 28 |
disruptive | 5 | 63 | 36 | 79 |
missense | 5 | 52 | 35 | 58 |
PlasmoDB Total SNPs: 110
Non-coding: 87 | Synonymous: 9 | Nonsynonymous: 12 | Stop Codon: 2
Protein Length: 274 | Molecular Weight (kDa): 31.735
UniProt ID(s): Q8IJV5
PDB ID(s): None
Isoelectric Point: 6.04
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR000878 | Tetrapyrrole methylase |
InterPro | IPR004551 | Diphthine synthase |
InterPro | IPR035996 | Tetrapyrrole methylase superfamily |
PFam | PF00590 | Tetrapyrrole methylase |
Superfamily | SSF53790 | Tetrapyrrole methylase superfamily |
PMID | Title | Authors | DOI/Link |
---|---|---|---|
12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
30335802 | Importance of diphthamide modified EF2 for translational accuracy and competitivecell growth in yeast | Hawer H, Utkur K, ..., Schaffrath R | 10.1371/journal.pone.0205870 |
37675463 | DPH1 and DPH2 variants that confer susceptibility to diphthamide deficiencysyndrome in human cells and yeast models | Utkur K, Mayer K, ..., Brinkmann U | 10.1242/dmm.050207 |
23468660 | The amidation step of diphthamide biosynthesis in yeast requires DPH6, a geneidentified through mining the DPH1-DPH5 interaction network | Uthman S, Bar C, ..., Schaffrath R | 10.1371/journal.pgen.1003334 |