PF3D7_1009300

Genome location: Pf3D7_10_v3:377,760..379,943(-)

Genome classification: Core

Function and Localization

Product Description: AMP-binding protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0031956	medium-chain fatty acid-CoA ligase activity
Process	GO:0006631	fatty acid metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.20	0.90
Trophozoite	expressed	0.37	0.77
Schizont	expressed	0.14	0.91
Gametocyte	expressed	0.39	0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1009300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.122 | MFS: -3.117 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJV2

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532399

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	11	13	36
disruptive	13	43	33	82
missense	7	41	31	79

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	36	21	40
disruptive	23	109	73	151
missense	10	92	65	116

PlasmoDB Total SNPs: 74

Non-coding: 37 | Synonymous: 28 | Nonsynonymous: 8 | Stop Codon: 1

Protein Information

Protein Length: 600 | Molecular Weight (kDa): 71.993

UniProt ID(s): Q8IJV2

PDB ID(s): None

Isoelectric Point: 9.24

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000873	AMP-dependent synthetase/ligase
PFam	PF00501	AMP-dependent synthetase/ligase
Superfamily	SSF56801	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21