PF3D7_1009500

Genome location: Pf3D7_10_v3:382,660..385,664(-)

Genome classification: Core

Function and Localization

Product Description: metalloprotease, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.24.- (Metalloendopeptidases.)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0008237	metallopeptidase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.10	0.95
Trophozoite	expressed	0.67	0.62
Schizont	possibly expressed	0.14	0.91
Gametocyte	expressed	0.64	0.64

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1009500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.211 | MFS: -2.685 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1207800)

Relative Growth Rate: 1.02 ± 0.06
Confidence: 6.87

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1207800)

Modification: Disrupted | RMgm-3202

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJV0

"Best" AlphaFill ligand hit: UDP (uridine-5'-diphosphate, Local RMSD=3.04) with 3HBN (Global RMSD=14.40)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.24.-	Ex: meprin A	GSHSDSATPADPNEMDTTPTXTNFEDTAEGTAPMSFPCMB...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_108834

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	13	12	27
disruptive	11	44	28	72
missense	10	44	27	65

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	35	12	28
disruptive	19	97	55	104
missense	14	86	51	78

PlasmoDB Total SNPs: 218

Non-coding: 185 | Synonymous: 24 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 375 | Molecular Weight (kDa): 43.788

UniProt ID(s): Q8IJV0

PDB ID(s): None

Isoelectric Point: 9.55

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR013536	WLM domain
PFam	PF08325	WLM domain
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21